概述

  • 产品名称Anti-WDR36抗体
    参阅全部 WDR36 一抗
  • 描述
    兔多克隆抗体to WDR36
  • 经测试应用适用于: WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from N terminal domain of human WDR36

性能

应用

Our Abpromise guarantee covers the use of ab73548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 105 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 相关性WDR36 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. WDR36 is involved in T cell activation and is highly co-regulated with IL2. Mutations in WDR36 are associated with adult-onset primary open-angle glaucoma (POAG).
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • DKFZp686I1650 antibody
    • GLC 1G antibody
    • GLC1G antibody
    • T cell activation WD repeat containing protein antibody
    • T cell activation WD repeat protein antibody
    • TA WDRP antibody
    • TAWDRP antibody
    • UTP 21 antibody
    • UTP21 antibody
    • UTP21, S. cerevisiae, homolog of antibody
    • WD repeat containing protein 36 antibody
    • WD repeat domain 36 antibody
    • WDR 36 antibody
    see all

Anti-WDR36 antibody 图像

  • ab73548 staining WDR36 in immortalized Human trabecular meshwork cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% BSA for 45 minutes. The primary antibody was diluted 1/50 in 0.3% BSA/PBS and incubated with the sample for 2 hours. The secondary antibody was Alexa Fluor 488® conjugated goat anti-rabbit polyclonal, diluted 1/300.

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Anti-WDR36 antibody (ab73548)参考文献

This product has been referenced in:
  • Gallenberger M  et al. Lack of WDR36 leads to preimplantation embryonic lethality in mice and delays the formation of small subunit ribosomal RNA in human cells in vitro. Hum Mol Genet 20:422-35 (2011). ICC/IF ; Human . Read more (PubMed: 21051332) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (immortalized human trabecular meshwork cells)
Specification immortalized human trabecular meshwork cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step BSA as blocking agent for 45 minute(s) · Concentration: 3%
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提交于 Feb 26 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"