概述

  • 产品名称Anti-WDR34抗体
    参阅全部 WDR34 一抗
  • 描述
    兔多克隆抗体to WDR34
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig, Xenopus laevis
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 431-480 (QLSLKYLFAV RWSPVRPLVF AAASGKGDVQ LFDLQKSSQK PTVLIKQTQD) of human WDR34 (NP_443076).

  • 阳性对照
    • Fetal Brain lysate. IF/ICC: HeLa cell line.

性能

应用

Our Abpromise guarantee covers the use of ab81030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

靶标

Anti-WDR34 antibody 图像

  • ICC/IF image of ab81030 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81030, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-WDR34 antibody (ab81030) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 12%

Anti-WDR34 antibody (ab81030)参考文献

ab81030 has not yet been referenced specifically in any publications.

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