Customer reviews and FAQs

概述

  • 产品名称Anti-WDR25抗体
    参阅全部 WDR25 一抗
  • 描述
    兔多克隆抗体to WDR25
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 12-120 of Human WDR25 (Q64LD2).

  • 阳性对照
    • RT4, U-251 MG, Human Plasma, Human Liver and Human Tonsil lysates; Human testis tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-WDR25 antibody 图像

  • Immunocytochemistry/ Immunofluorescence - Anti-WDR25 antibody (ab121170)
    Immunocytochemistry/ Immunofluorescence - Anti-WDR25 antibody (ab121170)
    Immunocytochemistry/immunofluorescence analysis of PC-3 cells labelling WDR25 with ab121170 shows positive staining in the nucleus, but excluded from the nucleoli.
  • Western blot - Anti-WDR25 antibody (ab121170)
    Western blot - Anti-WDR25 antibody (ab121170)
    All lanes : Anti-WDR25 antibody (ab121170) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR25 antibody (ab121170)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR25 antibody (ab121170)
    ab121170 at 1/75 dilution staining WDR25 in Paraffin-emebedded Human testis tissue by Immunohistochemistry.

Anti-WDR25 antibody (ab121170)参考文献

ab121170 has not yet been referenced specifically in any publications.

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