概述

  • 产品名称Anti-WDR20抗体
    参阅全部 WDR20 一抗
  • 描述
    兔多克隆抗体to WDR20
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Chicken, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residue 450 and 500 of Human WDR20 (AAH28387.1).

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab85729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 63 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-WDR20 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling WDR20 with at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-WDR20 antibody (ab85729) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,200 kDa,38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Human WDR20 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85729 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-WDR20 antibody (ab85729)参考文献

ab85729 has not yet been referenced specifically in any publications.

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