概述

  • 产品名称Anti-WDR20抗体
    参阅全部 WDR20 一抗
  • 描述
    小鼠单克隆抗体to WDR20
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human, Kangaroo, Non Human Primates, Snake, Macaque Monkey, Deer, Plasmodium falciparum, Staphylococcus aureus, Syrian Hamster, Dragon Fly, Electric Eel (Electrophorus electricus), Hoolock Gibbon, Lethocerus indicus (Waterbug)
  • 免疫原

    Recombinant full length (aa 1- 570) human WDR20 protein with tag (AAH28387)

  • 阳性对照
    • WDR20 transfected 293T cell lysate; recombinant WDR20.

性能

应用

Our Abpromise guarantee covers the use of ab68356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 序列相似性Contains 5 WD repeats.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp686B2131 antibody
      • DMR antibody
      • FLJ27314 antibody
      • FLJ33659 antibody
      • MGC33177 antibody
      • MGC33183 antibody
      • Protein DMR antibody
      • WD repeat domain 20 antibody
      • WD repeat ontaining protein 20 antibody
      • WD repeat protein 20 antibody
      • WD repeat-containing protein 20 antibody
      • WDR 20 antibody
      • Wdr20 antibody
      • WDR20_HUMAN antibody
      see all

    Anti-WDR20 antibody 图像

    • Anti-WDR20 antibody (ab68356) at 1/500 dilution + recombinant WDR20 at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 63 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-WDR20 antibody (ab68356) at 1/500 dilution

      Lane 1 : WDR20 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 63 kDa
      Observed band size : 63 kDa

    Anti-WDR20 antibody (ab68356)参考文献

    ab68356 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"