The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 137 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WDR11 is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. The WDR11 gene is located in the chromosome 10q25-26 region, which is frequently deleted in gliomas and tumors of other tissues, and is disrupted by the t(10;19) translocation rearrangement in glioblastoma cells. The gene location suggests that it is a candidate gene for the tumor suppressor locus.
Membrane; Single-pass membrane protein, Cytoplasmic and Nuclear
Bromodomain and WD repeat containing protein 2 antibody
Bromodomain and WD repeat domain containing 2 antibody
Bromodomain- and WD repeat-containing protein 2, formerly antibody
BRWD2, formerly antibody
WD repeat containing protein 11 antibody
WD repeat containing protein 15 antibody
WD repeat domain 11 antibody
WD repeat domain 15 antibody
WDR 11 antibody
Western blot - WDR11 antibody (ab93871)
All lanes : Anti-WDR11 antibody (ab93871) at 0.04 µg/ml
Lane 1 : Hela whole cell lysate at 50 µg Lane 2 : Hela whole cell lysate at 15 µg Lane 3 : Hela whole cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 137 kDa
Exposure time: 3 minutes
Immunoprecipitation - WDR11 antibody (ab93871)
ab93871 at 1 µg/ml detecting WDR11 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of WDR11
Lane 2: ab93871 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence an with exposure time of 10 seconds
Taylor KE & Mossman KL Cellular Protein WDR11 Interacts with Specific Herpes Simplex Virus Proteins at the trans-Golgi Network To Promote Virus Replication. J Virol89:9841-52 (2015).
Read more (PubMed: 26178983) »