概述

  • 产品名称Anti-WDHD1抗体
    参阅全部 WDHD1 一抗
  • 描述
    兔多克隆抗体to WDHD1
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    A synthetic peptide corresponding to a region between residues 1079 and 1129 of human WDHD1.

  • 阳性对照
    • HeLa whole cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab72436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500. Use with methanol fixed cells.
WB 1/2000 - 1/10000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
IP Use a concentration of 2 - 5 µg/ml.

靶标

  • 相关性The protein WDHD1 contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms.
  • 细胞定位Nuclear; nucleoplasm.
  • 数据库链接
  • 别名
    • Acidic nucleoplasmic DNA binding protein 1 antibody
    • And 1 antibody
    • And1 antibody
    • WD repeat and HMG box DNA binding protein 1 antibody
    • WDHD 1 antibody
    see all

Anti-WDHD1 antibody 图像

  • Immunoprecipitation of WDHD1 from 1mg whole HeLa cells lysate by 3 ug ab72436 followed by WB (20% of IP loaded):
    Lane 1: ab72429 precipitate

    Lane 2: ab72436 precipitate

    Lane 3: Control IgG

    For blotting immunoprecipitated WDHD1, ab72436 was used at 1 ug/ml.

    Detection: Chemiluminescence with an exposure time of 10 seconds.
  • All lanes : Anti-WDHD1 antibody (ab72436) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 126 kDa
    Observed band size : 126 kDa
  • ab72436 (1/500) staining WDHD1 in assynchronous HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). for further experimental details please refer to Abreview.

    See Abreview

Anti-WDHD1 antibody (ab72436)参考文献

This product has been referenced in:
  • Zhou Y  et al. Role of WDHD1 in Human Papillomavirus-Mediated Oncogenesis Identified by Transcriptional Profiling of E7-Expressing Cells. J Virol 90:6071-84 (2016). Read more (PubMed: 27099318) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

提交于 Mar 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"