The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use at a concentration of 2 - 5 µg/mg lysate.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性The protein WDHD1 contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms.
Acidic nucleoplasmic DNA binding protein 1 antibody
And 1 antibody
WD repeat and HMG box DNA binding protein 1 antibody
WDHD 1 antibody
Anti-WDHD1 antibody 图像
Immunoprecipitation - WDHD1 antibody (ab72429)
Immunoprecipitation of WDHD1 from 1mg whole HeLa cells lysate by 3 ug ab72429 followed by WB (20% of IP loaded):
Lane 1: ab72429 precipitate
Lane 2: ab72436 precipitate
Lane 3: Control IgG
For blotting immunoprecipitated WDHD1, ab72436 was used at 1 ug/ml.
Detection: ReliaBLOT® Reagents and Procedures were incorporated followed by chemiluminescence with an exposure time of 10 seconds.
Anti-WDHD1 antibody (ab72429)参考文献
has not yet been referenced specifically in any publications.