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Tissue/ cell preparation: Con A stimulated bovine lymphocytes.
This clone (CC101) immunoprecipitates a 215 kD molecule from bovine cells, and also recognises the swine homologue of WC1, which is a 180kD molecule. In pigs the 180kD molecule is expressed by a gamma/delta TCR positive T cell population that is also CD2, CD4, CD8. Weak results in flow cytometry.
Our Abpromise guarantee covers the use of ab34050 in the following tested applications.
|Flow Cyt||1/10 - 1/50.
Use 10µl of the suggested working dilution for 106 cells in 100µl.
ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/10 - 1/50.|
|IP||Use at an assay dependent concentration.|
ab34050 has not yet been referenced specifically in any publications.