The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: Use at a concentration of 0.2 - 0.4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 62 kDa). Staining of the WAVE band is specifically
inhibited with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
组织特异性Highly expressed in brain. Lowly expressed in testis, ovary, colon, kidney, pancreas, thymus, small intestine and peripheral blood.
序列相似性Belongs to the SCAR/WAVE family. Contains 1 WH2 domain.
结构域Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.
细胞定位Cytoplasm > cytoskeleton. Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas.
Payne LJ et al. p53 Down Regulates PDGF-Induced Formation of Circular Dorsal Ruffles in Rat Aortic Smooth Muscle Cells. PLoS One9:e108257 (2014).
Read more (PubMed: 25247424) »
Nascimento DS et al. Satellite Glial Cells Surrounding Primary Afferent Neurons Are Activated and Proliferate during Monoarthritis in Rats: Is There a Role for ATF3? PLoS One9:e108152 (2014).
Read more (PubMed: 25247596) »