重组
RabMAb

Anti-WASP抗体[EPR2540Y] (ab68182)

概述

  • 产品名称
    Anti-WASP抗体[EPR2540Y]
    参阅全部 WASP 一抗
  • 描述
    兔单克隆抗体[EPR2540Y] to WASP
  • 经测试应用
    适用于: WB, IP, ICC, Flow Cytmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues near the N terminus of human WASP

  • 阳性对照
    • TF1, U937 and Jurkat cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR2540Y
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab68182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能
      Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
    • 组织特异性
      Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
    • 疾病相关
      Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
      Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
      Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
    • 序列相似性
      Contains 1 CRIB domain.
      Contains 1 WH1 domain.
      Contains 1 WH2 domain.
    • 结构域
      The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
      The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
    • 细胞定位
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Eczema thrombocytopenia antibody
      • IMD2 antibody
      • SCNX antibody
      • THC antibody
      • THC1 antibody
      • Thrombocytopenia 1 (X linked) antibody
      • U42471 antibody
      • Was antibody
      • WASp antibody
      • WASP_HUMAN antibody
      • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
      • Wiskott Aldrich syndrome antibody
      • Wiskott Aldrich syndrome protein antibody
      • Wiskott-Aldrich syndrome protein antibody
      see all

    Anti-WASP antibody [EPR2540Y] 图像

    • All lanes : Anti-WASP antibody [EPR2540Y] (ab68182) at 1/2000 dilution

      Lane 1 : TF1 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 53 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

    Anti-WASP antibody [EPR2540Y] (ab68182)参考文献

    ab68182 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab68182.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册