RabMAb

Anti-WASP抗体[EP2541Y] (ab75830)

概述

  • 产品名称Anti-WASP抗体[EP2541Y]
    参阅全部 WASP 一抗
  • 描述
    兔单克隆抗体[EP2541Y] to WASP
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminal of human WASP.

  • 阳性对照
    • TF-1 and U937 cell lysates. Human spleen tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP2541Y
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
    • 组织特异性Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
    • 疾病相关Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
      Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
      Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
    • 序列相似性Contains 1 CRIB domain.
      Contains 1 WH1 domain.
      Contains 1 WH2 domain.
    • 结构域The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
      The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
    • 细胞定位Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Eczema thrombocytopenia antibody
      • IMD2 antibody
      • SCNX antibody
      • THC antibody
      • THC1 antibody
      • Thrombocytopenia 1 (X linked) antibody
      • U42471 antibody
      • Was antibody
      • WASp antibody
      • WASP_HUMAN antibody
      • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
      • Wiskott Aldrich syndrome antibody
      • Wiskott Aldrich syndrome protein antibody
      • Wiskott-Aldrich syndrome protein antibody
      see all

    Anti-WASP antibody [EP2541Y] 图像

    • All lanes : Anti-WASP antibody [EP2541Y] (ab75830) at 1/50000 dilution

      Lane 1 : TF-1 cell lysate
      Lane 2 : U937 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 53 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    • ab75830 at 1/100 dilution staining WASP in paraffin-embedded human spleen tissue.
    • Flow cytometry analysis of permeabilized U937 cells, staining WASP (red) with ab75830 at 1/10 dilution. Rabbit IgG was used as negative control (green).

    Anti-WASP antibody [EP2541Y] (ab75830)参考文献

    ab75830 has not yet been referenced specifically in any publications.

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