概述

  • 产品名称Anti-WASP抗体
    参阅全部 WASP 一抗
  • 描述
    兔多克隆抗体to WASP
  • 特异性ab59279 detects endogenous levels of total WASP protein.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthesized non phosphopeptide derived from human WASP around the phosphorylation site of tyrosine 290 (LIYPDF).

  • 阳性对照
    • Extracts from HepG2 cells (WB) Human brain tissue (IHC)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab59279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

靶标

  • 功能Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
  • 组织特异性Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
  • 疾病相关Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
    Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
    Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
  • 序列相似性Contains 1 CRIB domain.
    Contains 1 WH1 domain.
    Contains 1 WH2 domain.
  • 结构域The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
    The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Eczema thrombocytopenia antibody
    • IMD2 antibody
    • SCNX antibody
    • THC antibody
    • THC1 antibody
    • Thrombocytopenia 1 (X linked) antibody
    • U42471 antibody
    • Was antibody
    • WASp antibody
    • WASP_HUMAN antibody
    • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
    • Wiskott Aldrich syndrome antibody
    • Wiskott Aldrich syndrome protein antibody
    • Wiskott-Aldrich syndrome protein antibody
    see all

Anti-WASP antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab59279 at a dilution of 1/50. Left hand image - without immunizing peptide; right hand image - with immunizing peptide.
  • All lanes : Anti-WASP antibody (ab59279) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells with no immunizing peptide
    Lane 2 : Extracts from HepG2 cells with immunizing peptide


    Predicted band size : 53 kDa

Anti-WASP antibody (ab59279)参考文献

ab59279 has not yet been referenced specifically in any publications.

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