概述

  • 产品名称Anti-WASP抗体
    参阅全部 WASP 一抗
  • 描述
    山羊多克隆抗体to WASP
  • 特异性This antibody reacts Wiskott-Aldrich Syndrome Protein. It is not expected to cross-react with N WASP (WASL).
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    C-SPADKKRSGKK KI

    , corresponding to Internal sequence amino acids 220-232 of Human WASP

  • 阳性对照
    • U937 and MOLT4 cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab28769 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Detection limit 1/128,000,
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 53 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Effector protein for Rho-type GTPases, providing a link with the Arp2/3 complex that regulates the structure and dynamics of the actin cytoskeleton. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function.
    • 组织特异性Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.
    • 疾病相关Defects in WAS are the cause of Wiskott-Aldrich syndrome (WAS) [MIM:301000]; also known as eczema-thrombocytopenia-immunodeficiency syndrome. WAS is an X-linked recessive immunodeficiency characterized by eczema, thrombocytopenia, recurrent infections, and bloody diarrhea. Death usually occurs before age 10.
      Defects in WAS are the cause of thrombocytopenia type 1 (THC1) [MIM:313900]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.
      Defects in WAS are a cause of neutropenia severe congenital X-linked (XLN) [MIM:300299]. XLN is an immunodeficiency syndrome characterized by recurrent major bacterial infections, severe congenital neutropenia, and monocytopenia.
    • 序列相似性Contains 1 CRIB domain.
      Contains 1 WH1 domain.
      Contains 1 WH2 domain.
    • 结构域The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.
      The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.
    • 细胞定位Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Eczema thrombocytopenia antibody
      • IMD2 antibody
      • SCNX antibody
      • THC antibody
      • THC1 antibody
      • Thrombocytopenia 1 (X linked) antibody
      • U42471 antibody
      • Was antibody
      • WASp antibody
      • WASP_HUMAN antibody
      • Wiskott Aldrich syndrome (eczema thrombocytopenia) antibody
      • Wiskott Aldrich syndrome antibody
      • Wiskott Aldrich syndrome protein antibody
      • Wiskott-Aldrich syndrome protein antibody
      see all

    Anti-WASP antibody 图像

    • Anti-WASP antibody (ab28769) at 0.03 µg/ml + U937 cell lysate (total protein/per lane) at 30 µg/ml
      Developed using the ECL technique

      Predicted band size : 53 kDa
      Observed band size : 53-65 kDa (why is the actual band size different from the predicted?)

    Anti-WASP antibody (ab28769)参考文献

    ab28769 has not yet been referenced specifically in any publications.

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