WB, ICC/IF, IHC-P more details
Recombinant fragment corresponding to Human WASH4P aa 363-477.
GAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKKKQKEQEQV RATSQGGHLMSDLFNKLVMRHKGISGKGPGAGEGPGGAFARVSDSIPPLP PPQQPQVDEDEDDWES
Database link: A8MWX3
IHC-P: Human testis tissue
WB: Human RT-4 and U-251 MG cell lysates
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液 pH: 7.20 Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
相关性 Acts as a nucleation-promoting factor at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting.
Putative WAS protein family homolog 4 antibody
Anti-WASH4P antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WASH4P antibody (ab121151)
Immunohistochemical staining of WASH3P in Human testis tissue, using ab121151 at a 1/1000 dilution. The image shows strong nuclear positivity in cells of seminiferus ducts.
Western blot - Anti-WASH4P antibody (ab121151)
All lanes : Anti-WASH4P antibody (ab121151) at 1/250 dilution Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : A-431 cell lysate Lane 4 : Liver cell lysate Lane 5 : Tonsil cell lysate
Immunocytochemistry/ Immunofluorescence - Anti-WASH4P antibody (ab121151)
Immunofluorescent staining of Human cell line U-251MG shows positivity in vesicles using ab121151. Recommended treatment: Fixation/Permeabilization: PFA/Triton X-100
Anti-WASH4P antibody (ab121151)参考文献
has not yet been referenced specifically in any publications.
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