概述

  • 产品名称Anti-WAC抗体
    参阅全部 WAC 一抗
  • 描述
    兔多克隆抗体to WAC
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Cow, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human WAC aa 450-550 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9BTA9

  • 阳性对照
    • This antibody gave a positive signal in HeLa Nuclear lysate as well as the following whole cell lysates: HeLa; HEK293; NIH3T3. This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 71 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage.
  • 序列相似性Contains 1 WW domain.
  • 翻译后修饰Phosphorylated on tyrosine residues.
  • 细胞定位Nucleus speckle. Nucleus. In distinct nuclear speckles. Colocalizes with pre-mRNA processing complexes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110067P07Rik antibody
    • BA48B24 antibody
    • BA48B24.1 antibody
    • BM 016 antibody
    • FLJ31290 antibody
    • KIAA1844 antibody
    • MGC10753 antibody
    • OTTHUMP00000019380 antibody
    • OTTHUMP00000019382 antibody
    • PRO1741 antibody
    • wac antibody
    • WAC_HUMAN antibody
    • WW domain containing adapter protein with coiled coil antibody
    • WW domain containing adaptor with coiled coil antibody
    • WW domain-containing adapter protein with coiled-coil antibody
    • Wwp4 antibody
    see all

Anti-WAC antibody 图像

  • ICC/IF image of ab109486 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109486 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-WAC antibody (ab109486) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 71 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    The predicted molecular weight of WAC is71 kDa (SwissProt), however we expect to observe a banding pattern around 85 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab109486 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-WAC antibody (ab109486)参考文献

ab109486 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Jul 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"