Anti-WAC抗体(ab109486)
Key features and details
- Rabbit polyclonal to WAC
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-WAC抗体
参阅全部 WAC 一抗 -
描述
兔多克隆抗体to WAC -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
-
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Cow, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- This antibody gave a positive signal in HeLa Nuclear lysate as well as the following whole cell lysates: HeLa; HEK293; NIH3T3. This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109486于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 71 kDa).
|
|
ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 71 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
-
功能
Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. -
序列相似性
Contains 1 WW domain. -
翻译后修饰
Phosphorylated on tyrosine residues. -
细胞定位
Nucleus speckle. Nucleus. In distinct nuclear speckles. Colocalizes with pre-mRNA processing complexes. - Information by UniProt
-
数据库链接
- Entrez Gene: 51322 Human
- Entrez Gene: 225131 Mouse
- Omim: 615049 Human
- SwissProt: Q9BTA9 Human
- SwissProt: Q924H7 Mouse
- Unigene: 435610 Human
- Unigene: 204524 Mouse
- Unigene: 482081 Mouse
-
别名
- 1110067P07Rik antibody
- BA48B24 antibody
- BA48B24.1 antibody
see all
图片
-
ICC/IF image of ab109486 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109486 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
-
All lanes : Anti-WAC antibody (ab109486) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThe predicted molecular weight of WAC is71 kDa (SwissProt), however we expect to observe a banding pattern around 85 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab109486 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab109486 尚未被引用在任何文献中。