Anti-VSX1抗体(ab105818)

概述

  • 产品名称Anti-VSX1抗体
    参阅全部 VSX1 一抗
  • 描述
    兔多克隆抗体to VSX1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to a region within N-terminal amino acids 10 - 60 (RSRSRALAPG CPPTGSRLRS FAINDLLGLE ADLPTPAEPG LRSNSGDPAE) of Mouse VSX1 (NP_473409).

  • 阳性对照
    • Mouse spleen lysate

性能

应用

Our Abpromise guarantee covers the use of ab105818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 39 kDa.

Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Binds to the 37-bp core of the locus control region (LCR) of the red/green visual pigment gene cluster. May regulate the activity of the LCR and the cone opsin genes at earlier stages of development.
  • 组织特异性In the adult eye, expressed in lens, iris, ciliary body, choroid, optical nerve head and, most strongly, in retina, but not expressed in sclera and cornea. According to PubMed 11978762, expressed in adult retina but not in lens and cornea. Within adult retina, found exclusively in the inner nuclear layer. Isoform 1, isoform 2, isoform 3 and isoform 4 expressed in adult retina, but not in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. Not expressed in thymus and spleen. Expressed in embryonic craniofacial tissue. Expressed in fetal (week 14) retina. Strongly expressed in neonatal retina (day 0), weakly in neonatal lens (day 0), choroid (day 0) and cornea (day 0, 4; month 9).
  • 疾病相关Defects in VSX1 are a cause of posterior polymorphous corneal dystrophy type 1 (PPCD1) [MIM:122000]. PPCD1 is a slowly progressive hereditary disorder of the corneal endothelium that leads to a variable degree of visual impairment usually in adulthood.
    Defects in VSX1 are a cause of keratoconus type 1 (KTCN1) [MIM:148300]. Keratoconus type 1 is a frequent corneal dystrophy with an incidence that varies from 50 to 230 per 100'000. The cornea assumes a conical shape as a result of a progressive non-inflammatory thinning of the corneal stroma. Keratoconus is most often an isolated sporadic condition with cases of autosomal dominant and autosomal recessive transmission.
  • 序列相似性Belongs to the paired homeobox family.
    Contains 1 CVC domain.
    Contains 1 homeobox DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAASDS antibody
    • Homeodomain protein RINX antibody
    • KTCN antibody
    • KTCN1 antibody
    • Posterior polymorphous corneal dystrophy antibody
    • PPCD antibody
    • PPD antibody
    • Retinal inner nuclear layer homeobox protein antibody
    • RINX antibody
    • Transcription factor VSX1 antibody
    • Visual system homeobox 1 antibody
    • Visual system homeobox 1 homolog, CHX10 like antibody
    • Visual system homeobox 1 homolog, CHX10 like (zebrafish) antibody
    • VSX1 antibody
    • VSX1_HUMAN antibody
    see all

Anti-VSX1 antibody 图像

  • Anti-VSX1 antibody (ab105818) (0.2 - 1 ug/ml) + Mouse spleen tissue lysate

    Predicted band size : 39 kDa

Anti-VSX1 antibody (ab105818)参考文献

ab105818 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Embryonic spinal cord)
Specification Embryonic spinal cord
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate
Permeabilization Yes - 0.1% TritonX-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
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提交于 Feb 06 2013

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