Anti-VSV-G tag抗体[P5D4] (HRP) (ab49610)

概述

  • 产品名称Anti-VSV-G tag抗体[P5D4] (HRP)
    参阅全部 VSV-G tag 一抗
  • 描述
    小鼠单克隆抗体[P5D4] to VSV-G tag (HRP)
  • 偶联物HRP
  • 特异性This antibody recognizes native and denatured forms of VSV-G tagged proteins.In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.

  • 表位The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.
  • 阳性对照
    • Recombinant VSV-G-LacZ tagged fusion protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 0.164% Sodium phosphate, 1% BSA
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号P5D4
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 120 kDa.

靶标

  • 相关性Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • 别名
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Anti-VSV-G tag antibody [P5D4] (HRP) 图像

  • All lanes : Anti-VSV-G tag antibody [P5D4] (HRP) (ab49610) at 1/1000 dilution

    Lane 1 : recombinant VSV-G-LacZ tagged fusion protein at 0.05 µg
    Lane 2 : recombinant VSV-G-LacZ tagged fusion protein at 0.025 µg
    Lane 3 : recombinant VSV-G-LacZ tagged fusion protein at 0.01 µg
    Lane 4 : recombinant VSV-G-LacZ tagged fusion protein at 0.005 µg


    Observed band size : 120 kDa (why is the actual band size different from the predicted?)

Anti-VSV-G tag antibody [P5D4] (HRP) (ab49610)参考文献

This product has been referenced in:
  • Vega B  et al. Technical Advance: Surface plasmon resonance-based analysis of CXCL12 binding using immobilized lentiviral particles. J Leukoc Biol : (2011). WB . Read more (PubMed: 21593136) »

See 1 Publication for this product

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We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for...

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