概述

  • 产品名称Anti-VSV-G tag抗体[P5D4]
    参阅全部 VSV-G tag 一抗
  • 描述
    小鼠单克隆抗体[P5D4] to VSV-G tag
  • 经测试应用适用于: ICC/IF, WB, IP, ICCmore details
  • 种属反应性
    Reacts with VSV-G tag. Not yet tested in other species.
  • 免疫原

    Synthetic peptide corresponding to C terminal amino acids 497-511 of VSV-G tag conjugated to KLH

  • 阳性对照
    • Whole cell extracts expressing VSV-G tagged fusion protein.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR223940,  GR223940. Lot numbers other than GR223940 and GR223940 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    In infected cells, ab50549 localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.097% Sodium azide
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • Primary antibody说明In infected cells, ab50549 localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain.
  • 克隆单克隆
  • 克隆编号P5D4
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. PubMed: 21159872
WB 1/100000.
IP Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

靶标

  • 相关性Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • 别名
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

Anti-VSV-G tag antibody [P5D4] (ab50549)参考文献

This product has been referenced in:
  • Malikov V  et al. HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. Nat Commun 6:6660 (2015). WB . Read more (PubMed: 25818806) »
  • Lin AH  et al. Blockade of type I interferon (IFN) production by retroviral replicating vectors and reduced tumor cell responses to IFN likely contribute to tumor selectivity. J Virol 88:10066-77 (2014). Read more (PubMed: 24965455) »

See all 7 Publications for this product

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Unfortunately we have not tested this antibody with an N-terminal VSVG tagged protein. The epitope for this clone (P5D4) resides within the C-terminal five amino acids (NRLGK) of the 15-amino acid antigenic ...

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Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"