Anti-VSV-G tag抗体(ab83196)

概述

  • 产品名称
    Anti-VSV-G tag抗体
    参阅全部 VSV-G tag 一抗
  • 描述
    兔多克隆抗体to VSV-G tag
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    Reacts with Vesicular stomatitis virus.
  • 免疫原

    Full length protein

  • 常规说明
    0.2 µ filtered

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • 存储溶液
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 10 kDa.

靶标

  • 相关性
    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • 别名
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

图片

  • All lanes : Anti-VSV-G tag antibody (ab83196) at 1/1000 dilution

    Lane 1 : Human HEK293 cells transfected with pMD2.G (VSVG encoding construct) whole cell lysate (labelled +VSVG)
    Lane 2 : Human HEK293 mock transfected (labelled -VSVG)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-rabbit polyclonal HRP conjugate at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 10 kDa


    Exposure time : 10 seconds

    Image is courtesy of an anonymous AbReview.

    See Abreview

实验方案

文献

ab83196 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cell line)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
10 µg
Treatment
Transfected with pMD2.G or mock
Specification
HEK293 cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 28 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293 cell line)
Permeabilization
No
Specification
HEK293 cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 28 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册