概述

  • 产品名称
    Anti-VSV-G tag抗体
    参阅全部 VSV-G tag 一抗
  • 描述
    鸡多克隆抗体to VSV-G tag
  • 特异性
    This antibody recognises VSV-G tag.
  • 经测试应用
    适用于: ICC/IF, IP, WB, ELISAmore details
  • 免疫原

    Synthetic peptide: YTDIEMNRLGK, conjugated to KLH

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide.
    Constituents: PBS, pH 7.2.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was immunoaffinity purified off an antigen containing immunoabsorbant.
  • 克隆
    多克隆
  • 同种型
    IgY
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab27026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
WB 1/2000 - 1/10000.
ELISA 1/200 - 1/500.

靶标

  • 相关性
    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • 别名
    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

图片

  • ab27026 staining VSV-G tag in VSV-G-GFP overexpressing Hela cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilized in 0.1% saponin and blocked in 20mM glycine in PBS for 20 minutes at 23°C. A Alexa Fluor® 568 conjugated goat polyclonal to chicken was used, undiluted as secondary. Red color repersents staining with ab27026. 

    See Abreview

文献

This product has been referenced in:
  • Lopez CA  et al. Label-free multiplexed virus detection using spectral reflectance imaging. Biosens Bioelectron 26:3432-7 (2011). Read more (PubMed: 21342761) »
  • Salikhova A  et al. Vascular endothelial growth factor and semaphorin induce neuropilin-1 endocytosis via separate pathways. Circ Res 103:e71-9 (2008). ICC/IF . Read more (PubMed: 18723443) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for...

Read More

Thank you for your enquiry and your interest in our products.
This antibody recognizes VSV-G tag and it has been raised using a synthetic peptide: YTDIEMNRLGK - conjugated to KLH.
I would not suggest this antibody be used for any specific vir...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (human epithelial cell line)
Total protein in input
100 µg
Specification
human epithelial cell line
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

提交于 May 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cells overexpressing VSV-G-GFP)
Specification
HeLa cells overexpressing VSV-G-GFP
Fixative
Formaldehyde
Permeabilization
Yes - saponine 0.1%
Blocking step
20 mM glycine in PBS as blocking agent for 20 minute(s) · Concentration: 1500µg/mL · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Apr 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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