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Our Abpromise guarantee covers the use of ab27026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|WB||1/2000 - 1/10000.|
|ELISA||1/200 - 1/500.|
ab27026 staining VSV-G tag in VSV-G-GFP overexpressing Hela cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilized in 0.1% saponin and blocked in 20mM glycine in PBS for 20 minutes at 23°C. A Alexa Fluor® 568 conjugated goat polyclonal to chicken was used, undiluted as secondary. Red color repersents staining with ab27026.