Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide within residues: HRENVFLSYQ DKRINHGSLP HLQQRVRFAA SDPSQYDASI NLMNLQVSDT , corresponding to N terminal amino acids 72-121 of Human VSIG8 (NP_001013683).
- Human fetal muscle lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.
Information by UniProt
- C1orf204 antibody
- Chromosome 1 open reading frame 204 antibody
- EG240916 antibody
Western blot - VSIG8 antibody (ab83852)
Anti-VSIG8 antibody (ab83852) at 1 µg/ml (5% skim milk / PBS buffer) + Human fetal muscle lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
44 kDaObserved band size:
40 kDa (why is the actual band size different from the predicted?
Gel concentration: 12%
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"