• 产品名称Anti-VSIG4抗体
    参阅全部 VSIG4 一抗
  • 描述
    兔多克隆抗体to VSIG4
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: SHYTCEVTWQ TPDGNQVVRD KITELRVQKL SVSKPTVTTG SGYGFTVPQG, corresponding to N terminal amino acids 109-158 of Human VSIG4

  • 阳性对照
    • Jurkat cell lyaste.



Our Abpromise guarantee covers the use of ab56037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.


  • 功能Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.
  • 组织特异性Abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages.
  • 序列相似性Contains 2 Ig-like (immunoglobulin-like) domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Complement receptor of the immunoglobulin superfamily antibody
    • CRIg antibody
    • Ig superfamily protein antibody
    • Protein Z39Ig antibody
    • UNQ317/PRO362 antibody
    • V set and immunoglobulin domain containing 4 antibody
    • V-set and immunoglobulin domain-containing protein 4 antibody
    • VSIG4 antibody
    • VSIG4_HUMAN antibody
    • Z39IG antibody
    see all

Anti-VSIG4 antibody 图像

  • Anti-VSIG4 antibody (ab56037) at 5 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

Anti-VSIG4 antibody (ab56037)参考文献

ab56037 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions Reduced Denaturing (4-15% gradient)
Loading amount 20 µg
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Julie Kim

Verified customer

提交于 May 12 2017

Application Flow Cytometry
Sample Human Cell (monocytes from PBMC, Jurkat, THP-1, HEK293)
Permeabilization No
Gating Strategy CD14+ monocytes from PBMC respective cell line (see above)
Specification monocytes from PBMC, Jurkat, THP-1, HEK293
Preparation Cell harvesting/tissue preparation method: Monocytes were stained within PBMC which was isolated by density gradient centrifugation of whole blood Jurkat, THP-1 and HEK293 cell line were harvested out of cell culture flasks and resuspended in FACS Azide buffer
Sample buffer: FACS Azide buffer was used consisting of PBS + 2 mM EDTA + 0,1% Natriumazid + 2% FCS
Fixation no fixation

Abcam user community

Verified customer

提交于 Apr 13 2015

Expiration date: **********

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