Anti-VSIG4抗体(ab56037)

概述

  • 产品名称Anti-VSIG4抗体
    参阅全部 VSIG4 一抗
  • 描述
    兔多克隆抗体to VSIG4
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: SHYTCEVTWQ TPDGNQVVRD KITELRVQKL SVSKPTVTTG SGYGFTVPQG, corresponding to N terminal amino acids 109-158 of Human VSIG4

  • 阳性对照
    • Jurkat cell lyaste.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab56037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

  • 功能Phagocytic receptor, strong negative regulator of T-cell proliferation and IL2 production. Potent inhibitor of the alternative complement pathway convertases.
  • 组织特异性Abundantly expressed in several fetal tissues. In adult tissues, highest expression in lung and placenta. Expressed in resting macrophages.
  • 序列相似性Contains 2 Ig-like (immunoglobulin-like) domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Complement receptor of the immunoglobulin superfamily antibody
    • CRIg antibody
    • Ig superfamily protein antibody
    • Protein Z39Ig antibody
    • UNQ317/PRO362 antibody
    • V set and immunoglobulin domain containing 4 antibody
    • V-set and immunoglobulin domain-containing protein 4 antibody
    • VSIG4 antibody
    • VSIG4_HUMAN antibody
    • Z39IG antibody
    see all

Anti-VSIG4 antibody 图像

  • Anti-VSIG4 antibody (ab56037) at 5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

Anti-VSIG4 antibody (ab56037)参考文献

ab56037 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Sample Human Cell (monocytes from PBMC, Jurkat, THP-1, HEK293)
Permeabilization No
Gating Strategy CD14+ monocytes from PBMC respective cell line (see above)
Specification monocytes from PBMC, Jurkat, THP-1, HEK293
Preparation Cell harvesting/tissue preparation method: Monocytes were stained within PBMC which was isolated by density gradient centrifugation of whole blood Jurkat, THP-1 and HEK293 cell line were harvested out of cell culture flasks and resuspended in FACS Azide buffer
Sample buffer: FACS Azide buffer was used consisting of PBS + 2 mM EDTA + 0,1% Natriumazid + 2% FCS
Fixation no fixation
Username

Abcam user community

Verified customer

提交于 Apr 13 2015

DISCOUNT CODE: ********
Expiration date: **********

I am very pleased to hear you would like to accept our offer and test ab56037 in Flow Cytometry. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem ...

Read More

Thank you for your reply.

It is ok for your tech to so the ordering, but I will either need to place the code under their name, or if you could please let me know the institution that you work for, I can place that on the discount code notes....

Read More

Thank you very much for your interest in ab56037.

To our knowledge, ab56037 has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy ab56037 now, test it in Flow Cytometry and submit feedback ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"