概述

  • 产品名称
  • 描述
    兔多克隆抗体to VR1
  • 特异性
    Immunohistochemical analysis of rat dorsal root ganglia indicates a good specificity for this serum. Cross reactivity: This antibody is known to react with human and rat. Other species have not been tested.
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    CGSLKPEDAEVFKDSMVPGEK

    conjugated to KLH by a Glutaraldehyde linker, corresponding to C terminal amino acids 819-838 of Rat Vanilloid Receptor 1.

  • 阳性对照
    • IHC-Fr: Mouse rectum
  • 常规说明
    This antibody will stain a selected population of sensory neurons in the dorsal root ganglion. It is unsuitable for staining nerve fibres or terminals.

性能

应用

Our Abpromise guarantee covers the use of ab6166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Predicted molecular weight: 94,976 kDa.
ICC/IF 1/50 - 1/100.
IHC-Fr 1/50 - 1/100.

See Peier et al.

靶标

  • 功能
    Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • 组织特异性
    Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • 序列相似性
    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • 结构域
    The association domain (AD) is necessary for self-association.
  • 翻译后修饰
    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • 细胞定位
    Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Capsaicin receptor antibody
    • DKFZp434K0220 antibody
    • osm 9 like TRP channel 1 antibody
    • Osm-9-like TRP channel 1 antibody
    • OTRPC1 antibody
    • Transient receptor potential cation channel subfamily V member 1 antibody
    • TRPV 1 antibody
    • Trpv1 antibody
    • TRPV1_HUMAN antibody
    • Vanilloid receptor 1 antibody
    • Vanilloid receptor subtype 1 antibody
    • VR 1 antibody
    • VR1 antibody
    see all

Anti-VR1 antibody 图像

  • Immunohistochemical (Frozen) analysis of mouse rectum tissue section labeling VR1 with ab6166 at 1/50 diilution. The tissue was fixed with Paraformaldehyde and permeabilized with 5% acetone and 0.1% Triton. ab150077 at 1/200 was used as the secondary antibody.

    See Abreview

Anti-VR1 antibody (ab6166)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Rectum)
Permeabilization
Yes - After antigen retrival and a PBS wash (3X), 5% ice cold acetone was applied for 5 minutes, slides were then washed with PBS (3x) and 0.1% Triton was applied for 15 minutes.
Specification
Rectum
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Donna Daly

Verified customer

提交于 Sep 06 2017

I'm happy to help! Please let me know if you need anything further.

Thanks for the additional information.

I have located the order on our system and have arranged for a replacement vial of ab6166 to be included in Abcam's next shipment to you.

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Thank you for your enquiry.

I am sorry to hear of your customer's troubles using ab6166. I have read the attached complaint report and it does appear the most recent vial of ab6166 is not working as expected. I would be happy to offer a repla...

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Thank you for your enquiry and interest. This antibody is not purified and as the datasheet indicates we sell it as whole antiserum. ab6166 is a polyclonal antibody and there is batch-to-batch variation; some bleed may contain higher levels of red bloo...

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This antibody was raised against the intra-cellular C-terminal.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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