概述

  • 产品名称Anti-VR1抗体
    参阅全部 VR1 一抗
  • 描述
    兔多克隆抗体to VR1
  • 特异性Immunohistochemical staining of VR1 in human glabrous skin using this antibody results in the staining of epidermal and dermal cells, as well as free nerve fibers and Merkel cells. Immunofluorescent staining of VR1 in human prostatic urethra results in fibrous staining within the epithelial cells.
  • 经测试应用适用于: IHC-Fr, IHC-P, WB, ICC/IF, IHC-FrFlmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human VR1 aa 7-21.
    Sequence:

    TDLGAAADPLQKDTC


    (Peptide available as ab5894)

  • 阳性对照
    • IHC: human glabrous skin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明Vanilloid Receptor 1 (VR1), also known as capsaicin receptor, responds to both capsaicin, the pungent ingredient in hot peppers, and thermal stimuli within the noxious temperature range. This receptor is structurally related to the TRP family of ion channels and is predicted to contain 6 trans-membrane domains, a P-loop membrane domain and 3 ankyrin repeat domains. VR1 is highly permeable to divalent cations, including calcium and magnesium, as well as the monovalent cations sodium and potassium. Expression of VR1 appears to be limited to neurons within the sensory ganglia.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.Can be blocked with VR1 peptide (ab5894).
ICC/IF Use a concentration of 1 µg/ml.
IHC-FrFl Use at an assay dependent concentration.

靶标

  • 功能Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • 组织特异性Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • 序列相似性Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • 结构域The association domain (AD) is necessary for self-association.
  • 翻译后修饰Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • 细胞定位Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Capsaicin receptor antibody
    • DKFZp434K0220 antibody
    • osm 9 like TRP channel 1 antibody
    • Osm-9-like TRP channel 1 antibody
    • OTRPC1 antibody
    • Transient receptor potential cation channel subfamily V member 1 antibody
    • TRPV 1 antibody
    • Trpv1 antibody
    • TRPV1_HUMAN antibody
    • Vanilloid receptor 1 antibody
    • Vanilloid receptor subtype 1 antibody
    • VR 1 antibody
    • VR1 antibody
    see all

Anti-VR1 antibody 图像

  • Immunohistochemistry (frozen sections) analysis of human skin tissue sections labeling VR1 (red) with ab3487 at 1/250. A biotin-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

  • Immunolocalization of VR1 in human glabrous skin using ab3487.

Anti-VR1 antibody (ab3487)参考文献

This product has been referenced in:
  • Deering-Rice CE  et al. Transient receptor potential vanilloid-1 (TRPV1) is a mediator of lung toxicity for coal fly ash particulate material. Mol Pharmacol 81:411-9 (2012). WB . Read more (PubMed: 22155782) »
  • Denda S  et al. Glycolic acid induces keratinocyte proliferation in a skin equivalent model via TRPV1 activation. J Dermatol Sci 57:108-13 (2010). IHC-Fr ; Human . Read more (PubMed: 20060270) »

See all 2 Publications for this product

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Thank your email. I am very sorry that the suggestions did not helpto improve the results. I must therefore conclude, that the vial you received must have been damaged - maybe during storage or transport. I would be pleased to replace this vial for you...

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Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

We do not have a specific protocol for this antibody. We expect this antibody to work with a general IHC protocol after the classic opt...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin, 3D skin model)
Specification skin, 3D skin model
Fixative Methanol
Permeabilization Yes - 0.4% Triton X100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Human Tissue sections (skin)
Specification skin
Username

Abcam user community

Verified customer

提交于 Jun 10 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"