概述

  • 产品名称Anti-VR1抗体
    参阅全部 VR1 一抗
  • 描述
    豚鼠多克隆抗体to VR1
  • 经测试应用适用于: IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Guinea pig
  • 免疫原

    Synthetic peptide:

    YTGSLKPEDAEVFKDSMVPGEK

    , corresponding to C terminal amino acids 817-838 of Rat Vanilloid Receptor 1.

  • 阳性对照
    • This antibody gave a positive result when used in the following methanol fixed cell lines: PC12
  • 常规说明


    Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: Whole Serum
  • 纯度Whole antiserum
  • Primary antibody说明Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab10295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/1000.
ICC/IF 1/100.

靶标

  • 功能Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.
  • 组织特异性Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
  • 序列相似性Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.
  • 结构域The association domain (AD) is necessary for self-association.
  • 翻译后修饰Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
  • 细胞定位Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Capsaicin receptor antibody
    • DKFZp434K0220 antibody
    • osm 9 like TRP channel 1 antibody
    • Osm-9-like TRP channel 1 antibody
    • OTRPC1 antibody
    • Transient receptor potential cation channel subfamily V member 1 antibody
    • TRPV 1 antibody
    • Trpv1 antibody
    • TRPV1_HUMAN antibody
    • Vanilloid receptor 1 antibody
    • Vanilloid receptor subtype 1 antibody
    • VR 1 antibody
    • VR1 antibody
    see all

Anti-VR1 antibody 图像

  • ab10295 stained PC12 cells. The cells were 100% Methanol fixed for 5 minutes and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10295 at 1in100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab96959 Dylight 488 goat anti Guinea pig used at a 1/250 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab10295, at a dilution of 1/1000, staining VR1 in rat dorsal root ganglion by Immunohistochemistry (PFA perfusion fixed frozen sections).

Anti-VR1 antibody (ab10295)参考文献

This product has been referenced in:
  • Maione S  et al. Functional interaction between TRPV1 and micro-opioid receptors in the descending antinociceptive pathway activates glutamate transmission and induces analgesia. J Neurophysiol 101:2411-22 (2009). Read more (PubMed: 19297510) »
  • Naeini RS  et al. An N-terminal variant of Trpv1 channel is required for osmosensory transduction. Nat Neurosci 9:93-8 (2006). Read more (PubMed: 16327782) »

See all 2 Publications for this product

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I am happy to confirm that the blocking peptide for ab10295 is now available under the product number ab134720:

http://www.abcam.com/index.html?datasheet=134720 (or use the following: http://www.abcam.com/index.html?datasheet=134720...

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Thank you very much for providing that extra information. It has helped greatly in understanding the problems encountered and the optimisation you have already performed.

The anti-VR1 antibody (ab10295) has only been validated in frozen secti...

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Thank you for contacting us and reporting the problems you have encountered in using the anti-VR1 antibody (ab10295). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I ap...

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DISCOUNT CODE: ****** Expiration date: ***** I am very pleased to hear you would like to accept our offer and test ab10295 in western blot. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit ...

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Thank you for contacting Abcam about ab10295. We do not sell sample sizes of our antibodies but since ab10295  has not been tested in western blot, I can offer a discount off a future purchase if you buy ab10295 now, test it in western blot and submit ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
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提交于 Feb 13 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"