Full length human protein (AAH41868, aa 1 - 824)
VPS54-transfected 293T cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein G purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 110 kDa (SwissProt isoform 1).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
VPS54 belongs to the VPS54 family. In yeast VPS54 is part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. There are five named isoforms.
Hepatocellular carcinoma protein 8 antibody
Western blot - Anti-VPS54 antibody (ab69344)
All lanes :
Anti-VPS54 antibody (ab69344) at 1/500 dilution
Lane 1 :
VPS54 transfected 293T cell lysate
Lane 2 :
Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary All lanes :
Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution
Predicted band size:
Observed band size:
140 kDa (
why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"