Anti-VPS53抗体(ab106469)
Key features and details
- Rabbit polyclonal to VPS53
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-VPS53抗体
参阅全部 VPS53 一抗 -
描述
兔多克隆抗体to VPS53 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, ICCmore details -
种属反应性
与反应: Human -
免疫原
An 18 amino acid synthetic peptide from near the C terminus of Human VPS53 (EAW90658).
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阳性对照
- 293 cells and cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106469于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 80 kDa.
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ICC |
Use a concentration of 5 µg/ml.
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说明 |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 80 kDa. |
ICC
Use a concentration of 5 µg/ml. |
靶标
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功能
May be involved in retrograde transport of early and late endosomes to the late Golgi. -
序列相似性
Belongs to the VPS53 family. -
细胞定位
Golgi apparatus > trans-Golgi network membrane. Endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 55275 Human
- GenBank: BC006116 Human
- SwissProt: B3FH42 Human
- SwissProt: Q5VIR6 Human
- Unigene: 461819 Human
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别名
- FLJ10979 antibody
- FLJ41112 antibody
- FLJ61757 antibody
see all
图片
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Immunofluorescence of VPS53 in 293 cells with ab106469 at 2.5 ug/mL.
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Lane 1 : Anti-VPS53 antibody (ab106469) at 0.5 µg/ml
Lane 2 : Anti-VPS53 antibody (ab106469) at 1 µg/ml
All lanes : 293 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 80 kDa -
ab106469, at 5 µg/ml, staining VPS53 in 293 cells by Immunocytochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab106469 被引用在 2 文献中.
- Zhang Z et al. GZFLW Induces Apoptosis of Ectopic Endometrial Stromal Cells via Promoting VPS53 Protein Stability. Evid Based Complement Alternat Med 2018:1293630 (2018). PubMed: 30643524
- Masschaele D et al. RNF41 interacts with the VPS52 subunit of the GARP and EARP complexes. PLoS One 12:e0178132 (2017). ICC/IF . PubMed: 28542518