概述

  • 产品名称Anti-VPS4B抗体
    参阅全部 VPS4B 一抗
  • 描述
    兔多克隆抗体to VPS4B
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 350 and 400 of human VPS4B (NP_004860.2).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab86303 was affinity purified using an epitope specific to VPS4B immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5 µg/mg of lysate.
    WB: 1/2000 - 1/10,000. Predicted molecular weight: 49 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
    • 组织特异性Ubiquitously expressed.
    • 序列相似性Belongs to the AAA ATPase family.
      Contains 1 MIT domain.
    • 结构域The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
    • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 细胞定位Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 8030489C12Rik antibody
      • Cell migration inducing 1 antibody
      • Cell migration-inducing gene 1 protein antibody
      • MGC116271 antibody
      • MIG1 antibody
      • Protein SKD1 antibody
      • Skd1 antibody
      • SKD1B antibody
      • Suppressor of K(+) transport growth defect 1 antibody
      • Suppressor of K+ transport defect 1 antibody
      • Vacuolar protein sorting 4 homolog B (S. cerevisiae) antibody
      • Vacuolar protein sorting 4 homolog B antibody
      • Vacuolar protein sorting 4b antibody
      • Vacuolar protein sorting-associated protein 4B antibody
      • Vacuolar protein sorting-associating protein 4B antibody
      • VPS4 2 antibody
      • VPS42 antibody
      • Vps4b antibody
      • VPS4B_HUMAN antibody
      see all

    Anti-VPS4B antibody 图像

    • All lanes : Anti-VPS4B antibody (ab86303) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 49 kDa
      Observed band size : 49 kDa


      Exposure time : 1 minute
    • Detection of VPS4B by Western Blot of Immunprecipitate.
      ab86303, at 1 µg/ml, staining VPS4B in HeLa whole cell lysate immunoprecipitated using ab86303 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    Anti-VPS4B antibody (ab86303)参考文献

    ab86303 has not yet been referenced specifically in any publications.

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