Anti-VPS4B抗体(ab81416)

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概述

  • 产品名称
    Anti-VPS4B抗体
    参阅全部 VPS4B 一抗
  • 描述
    兔多克隆抗体to VPS4B
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human VPS4B.

    参阅Abcam的专有抗源政策

    (Peptide available as ab90848.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; Raji; Saos 2.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).

靶标

  • 功能
    Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the AAA ATPase family.
    Contains 1 MIT domain.
  • 结构域
    The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
  • Information by UniProt
  • 数据库链接
    • 别名
      • 8030489C12Rik antibody
      • Cell migration inducing 1 antibody
      • Cell migration-inducing gene 1 protein antibody
      • MGC116271 antibody
      • MIG1 antibody
      • Protein SKD1 antibody
      • Skd1 antibody
      • SKD1B antibody
      • Suppressor of K(+) transport growth defect 1 antibody
      • Suppressor of K+ transport defect 1 antibody
      • Vacuolar protein sorting 4 homolog B (S. cerevisiae) antibody
      • Vacuolar protein sorting 4 homolog B antibody
      • Vacuolar protein sorting 4b antibody
      • Vacuolar protein sorting-associated protein 4B antibody
      • Vacuolar protein sorting-associating protein 4B antibody
      • VPS4 2 antibody
      • VPS42 antibody
      • Vps4b antibody
      • VPS4B_HUMAN antibody
      see all

    图片

    • Western blot - VPS4B antibody (ab81416)
      Western blot - VPS4B antibody (ab81416)
      All lanes : Anti-VPS4B antibody (ab81416) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
      Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
      Lane 6 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 49 kDa
      Observed band size: 56 + 58 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute


      The 56 and 58 kDa band observed are comparable to molecular weights seen with other commercially available antibodies to Human Vacuolar protein sorting-associating protein 4B (VPS4B).

    实验方案

    文献

    ab81416 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab81416？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    1 Review or Q&A

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-VPS4B antibody

     Poor
    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100
    Read More
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    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Aug 03 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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