概述
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产品名称Anti-VPS4B抗体
参阅全部 VPS4B 一抗 -
描述兔多克隆抗体to VPS4B
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经测试应用适用于: WBmore details
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种属反应性与反应: Human
预测可用于: Mouse, Orangutan
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human VPS4B.
(Peptide available as ab90848.)
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; Raji; Saos 2.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab81416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa). |
靶标
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功能Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
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组织特异性Ubiquitously expressed.
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序列相似性Belongs to the AAA ATPase family.
Contains 1 MIT domain. -
结构域The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
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翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
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细胞定位Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
- Information by UniProt
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数据库链接
- Entrez Gene: 9525 Human
- Entrez Gene: 20479 Mouse
- Omim: 609983 Human
- SwissProt: O75351 Human
- SwissProt: P46467 Mouse
- Unigene: 126550 Human
- Unigene: 18705 Mouse
- Unigene: 483448 Mouse
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别名
- 8030489C12Rik antibody
- Cell migration inducing 1 antibody
- Cell migration-inducing gene 1 protein antibody
see all
Anti-VPS4B antibody 图像
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Western blot - VPS4B antibody (ab81416)All lanes : Anti-VPS4B antibody (ab81416) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 49 kDa
Observed band size : 56 + 58 kDa (why is the actual band size different from the predicted?)
Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 1 minute
Anti-VPS4B antibody (ab81416)参考文献
ab81416 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"