The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/3000. Predicted molecular weight: 49 kDa.
功能Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
序列相似性Belongs to the AAA ATPase family. Contains 1 MIT domain.
结构域The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
细胞定位Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
Suppressor of K(+) transport growth defect 1 antibody
Suppressor of K+ transport defect 1 antibody
Vacuolar protein sorting 4 homolog B (S. cerevisiae) antibody
Vacuolar protein sorting 4 homolog B antibody
Vacuolar protein sorting 4b antibody
Vacuolar protein sorting-associated protein 4B antibody
Vacuolar protein sorting-associating protein 4B antibody
VPS4 2 antibody
Anti-VPS4B antibody 图像
Western blot - Anti-VPS4B antibody (ab102687)
Anti-VPS4B antibody (ab102687) at 1/3000 dilution + 293T whole cell lysate at 30 µg
Predicted band size : 49 kDa
Anti-VPS4B antibody (ab102687)参考文献
This product has been referenced in:
Shtanko O et al. Crimean-Congo hemorrhagic fever virus entry into host cells occurs through the multivesicular body and requires ESCRT regulators. PLoS Pathog10:e1004390 (2014).
Read more (PubMed: 25233119) »