概述

  • 产品名称
  • 描述
    兔多克隆抗体to VPS4B
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 176-403 of Human VPS4B.

  • 阳性对照
    • 293T whole cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1.21% Tris, 0.75% Glycine
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab102687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/3000. Predicted molecular weight: 49 kDa.

靶标

  • 功能
    Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the AAA ATPase family.
    Contains 1 MIT domain.
  • 结构域
    The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 8030489C12Rik antibody
    • Cell migration inducing 1 antibody
    • Cell migration-inducing gene 1 protein antibody
    • MGC116271 antibody
    • MIG1 antibody
    • Protein SKD1 antibody
    • Skd1 antibody
    • SKD1B antibody
    • Suppressor of K(+) transport growth defect 1 antibody
    • Suppressor of K+ transport defect 1 antibody
    • Vacuolar protein sorting 4 homolog B (S. cerevisiae) antibody
    • Vacuolar protein sorting 4 homolog B antibody
    • Vacuolar protein sorting 4b antibody
    • Vacuolar protein sorting-associated protein 4B antibody
    • Vacuolar protein sorting-associating protein 4B antibody
    • VPS4 2 antibody
    • VPS42 antibody
    • Vps4b antibody
    • VPS4B_HUMAN antibody
    see all

图片

  • Anti-VPS4B antibody (ab102687) at 1/3000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 49 kDa

实验方案

文献

This product has been referenced in:
  • Shtanko O  et al. Crimean-Congo hemorrhagic fever virus entry into host cells occurs through the multivesicular body and requires ESCRT regulators. PLoS Pathog 10:e1004390 (2014). Human . Read more (PubMed: 25233119) »

See 1 Publication for this product

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