概述

  • 产品名称Anti-VPS4a抗体
    参阅全部 VPS4a 一抗
  • 描述
    兔多克隆抗体to VPS4a
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (VEYFLHAIKY EAHSDKAKES IRAKCVQYLD RAEKLKDYLR SKEKHGKKPV) of human VPS4a (NP_037377).

  • 阳性对照
    • Fetal muscle lysate.

性能

应用

Our Abpromise guarantee covers the use of ab81697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the AAA ATPase family.
    Contains 1 MIT domain.
  • 结构域The MIT domain serves as an adapter for ESCRT-III proteins. It forms an asymmetric three-helix bundle that binds amphipathic MIM (MIT interacting motif) helices along the groove between MIT helices 2 and 3 present in a subset of ESCRT-III proteins thus establishing the canonical MIM-MIT interaction. In an extended conformation along the groove between helices 1 and 3, also binds to a type-2 MIT interacting motif (MIM2).
  • 细胞定位Prevacuolar compartment membrane. Late endosome membrane. Membrane-associated in the prevacuolar endosomal compartment. Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hVPS4 antibody
    • Protein SKD2 antibody
    • SKD1 antibody
    • SKD1 homolog antibody
    • SKD1A antibody
    • SKD2 antibody
    • Vacuolar protein sorting 4 homolog A antibody
    • Vacuolar protein sorting 4, yeast, homolog of, A antibody
    • Vacuolar protein sorting factor 4A antibody
    • Vacuolar protein sorting-associated protein 4A antibody
    • vacuolar sorting protein 4 antibody
    • VPS4 antibody
    • VPS4-1 antibody
    • Vps4a antibody
    • VPS4A_HUMAN antibody
    see all

Anti-VPS4a antibody 图像

  • Anti-VPS4a antibody (ab81697) at 1 µg/ml + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa

Anti-VPS4a antibody (ab81697)参考文献

This product has been referenced in:
  • Bleck M  et al. Temporal and spatial organization of ESCRT protein recruitment during HIV-1 budding. Proc Natl Acad Sci U S A 111:12211-6 (2014). WB . Read more (PubMed: 25099357) »

See 1 Publication for this product

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