概述

  • 产品名称
  • 描述
    山羊多克隆抗体to VPS29
  • 特异性
    This antibody is expected to recognise both reported isoforms
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性

    预测可用于: Mouse, Rat, Dog, Human
  • 免疫原

    Peptide with sequence CTGNLCTKESYDYLK, from the internal region of the protein sequence

  • 阳性对照
    • Human spleen lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 8 µg/ml. In paraffin embedded Human Uterus shows vesicular and membranous staining in the endometrial glands.
WB Use at an assay dependent dilution. Preliminary experiments gave an approx 60kDa band in Human Spleen lysates after 0.3µg/ml antibody staining (expected size : 20.9kDa). The 60kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size but we would welcome any feedback.

靶标

  • 功能
    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro).
  • 组织特异性
    Ubiquitous. Highly expressed in heart, lung, placenta, spleen, peripheral blood leukocytes, thymus, colon skeletal muscle, kidney and brain.
  • 序列相似性
    Belongs to the VPS29 family.
  • 细胞定位
    Cytoplasm. Membrane. Endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DC15 antibody
    • DC7 antibody
    • DKFZp564F0223 antibody
    • FLJ20492 antibody
    • hVPS29 antibody
    • PEP11 antibody
    • PEP11 homolog antibody
    • Retromer protein antibody
    • Vacuolar protein sorting 29 antibody
    • Vacuolar protein sorting 29 (yeast homolog) antibody
    • Vacuolar protein sorting 29 (yeast) antibody
    • Vacuolar protein sorting 29 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 29 homolog antibody
    • Vacuolar protein sorting associated protein 29 antibody
    • Vacuolar protein sorting-associated protein 29 antibody
    • Vacuolar sorting protein VPS29/PEP11 antibody
    • Vesicle protein sorting 29 antibody
    • VPS 29 antibody
    • VPS29 antibody
    • VPS29 retromer complex component antibody
    • VPS29_HUMAN antibody
    • x 007 protein antibody
    see all

图片

  • ab51972 at 3.8 µg/ml staining VPS29 in Human uterus tissue. Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody detected by AP-staining.

文献

ab51972 has not yet been referenced specifically in any publications.

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