概述

  • 产品名称
  • 描述
    山羊多克隆抗体to VPS28
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide: ESAYNAFNRFLHA, corresponding to C terminal amino acids 209-211 of Human VPS28.

  • 阳性对照
    • Human testis and brain lysates.
  • 常规说明


    This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明
    This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).Can be blocked with Human VPS28 peptide (ab23183).

靶标

  • 功能
    Component of the ESCRT-I complex, a regulator of vesicular trafficking process.
  • 序列相似性
    Belongs to the VPS28 family.
    Contains 1 VPS28 C-terminal domain.
    Contains 1 VPS28 N-terminal domain.
  • 细胞定位
    Cell membrane. Late endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ESCRT-I complex subunit VPS28 antibody
    • H-Vps28 antibody
    • Vacuolar protein sorting 28 antibody
    • Vacuolar protein sorting 28 (yeast) antibody
    • Vacuolar protein sorting 28 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 28 homolog antibody
    • Vacuolar protein sorting-associated protein 28 homolog antibody
    • VPS 28 antibody
    • Vps28 antibody
    • VPS28_HUMAN antibody
    • Yeast class E protein Vps28p homolog antibody
    see all

Anti-VPS28 antibody 图像



  • Predicted band size : 25 kDa


    ab10133 at 1µg/ml staining approximately 24kDa band of human VPS28 in human testis lysate by Western blot (ECL).
  • ab10133 (10µg/ml) staining of Human Testis by IHC-P. Microwaved antigen retrieval with Tris/EDTA buffer pH9 and HRP-staining.

实验方案

Anti-VPS28 antibody (ab10133)参考文献

This product has been referenced in:
  • Mikula M  et al. Genome-wide co-localization of active EGFR and downstream ERK pathway kinases mirrors mitogen-inducible RNA polymerase 2 genomic occupancy. Nucleic Acids Res 44:10150-10164 (2016). Read more (PubMed: 27587583) »

See 1 Publication for this product

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