概述

  • 产品名称Anti-VPS25抗体
    参阅全部 VPS25 一抗
  • 描述
    山羊多克隆抗体to VPS25
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Dog
  • 免疫原

    Synthetic peptide corresponding to Human VPS25 aa 20-29 (internal sequence).
    Sequence:

    C-QPNVDTRQKQ

  • 阳性对照
    • WB: Human liver and rat kidney tissue lysates. IHC-P: Human kidney tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab42500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 21 kDa.

靶标

  • 功能Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.
  • 组织特异性Expressed at the mRNA level in kidney, liver, pancreas, and placenta. Lower levels of expression are found in heart, skeletal muscle, brain and lung.
  • 序列相似性Belongs to the VPS25 family.
  • 细胞定位Cytoplasm. Endosome membrane. Nucleus > nucleoplasm. Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dermal papilla derived protein 9 antibody
    • Dermal papilla-derived protein 9 antibody
    • DERP9 antibody
    • EAP20 antibody
    • ELL associated protein of 20 kDa antibody
    • ELL-associated protein of 20 kDa antibody
    • ESCRT-II complex subunit VPS25 antibody
    • FAP20 antibody
    • HGNC:28122 antibody
    • hVps25 antibody
    • MGC10540 antibody
    • Vacuolar protein sorting 25 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 25 antibody
    • Vacuolar protein-sorting-associated protein 25 antibody
    • VPS25 antibody
    • VPS25_HUMAN antibody
    see all

Anti-VPS25 antibody 图像

  • Anti-VPS25 antibody (ab42500) at 0.5 µg/ml + Rat kidney tissue lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa

    Incubated with the primary antibody for 1 hour.

  • ab42500 (10µg/ml) staining human kidney by IHC-P. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

  • Anti-VPS25 antibody (ab42500) at 0.5 µg/ml + Human liver tissue lysate (in RIPA buffer). at 35 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour.

Anti-VPS25 antibody (ab42500)参考文献

ab42500 has not yet been referenced specifically in any publications.

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