Anti-VPS24抗体(ab49266)

概述

  • 产品名称Anti-VPS24抗体
    参阅全部 VPS24 一抗
  • 描述
    兔多克隆抗体to VPS24
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: RVVDRQIRDI QREEEKVKRS VKDAAKKGQK DVCIVLAKEM IRSRKAVSKL, corresponding to amino acids 28-77 of Human VPS24

  • 阳性对照
    • HepG2 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab49266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Required for sorting/trafficking of EGF receptor.
  • 组织特异性Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 序列相似性Belongs to the SNF7 family.
  • 结构域The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • 细胞定位Cytoplasm > cytosol. Membrane. Endosome. Late endosome membrane. Localizes to the midbody of dividing cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • VPS 24 antibody
    • VPS24 antibody
    • CGI 149 antibody
    • CGI149 protein antibody
    • Charged multivesicular body protein 3 antibody
    • CHMP3 antibody
    • CHMP3_HUMAN antibody
    • Chromatin modifying protein 3 antibody
    • Chromatin-modifying protein 3 antibody
    • HGNC:29865 antibody
    • hVps24 antibody
    • NEDF antibody
    • Neuroendocrine differentiation factor antibody
    • Vacuolar protein sorting 24 (yeast) antibody
    • Vacuolar protein sorting 24 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting associated protein 24 antibody
    • Vacuolar protein sorting-associated protein 24 antibody
    • vps24 antibody
    see all

Anti-VPS24 antibody 图像

  • Anti-VPS24 antibody (ab49266) at 0.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 -1/100,000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • IHC image of ab49266 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab49266, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-VPS24 antibody (ab49266)参考文献

ab49266 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 2 µg
Gel Running Conditions Reduced Denaturing (4-12% Gel)
Sample Rat Cell lysate - whole cell (Neurons)
Specification Neurons
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Aug 22 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"