Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 891-941 of Human VPS11 (NP_068375.3).
293T, Jurkat and HeLa whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab125083 was affinity purified using an epitope specific to VPS11 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
Use at 2-10 µg/mg of lysate.
May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Ubiquitous. Expression was highest in heart and low in lung.
Belongs to the VPS11 family.
Contains 1 clathrin repeat. Contains 1 RING-type zinc finger.
Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
Information by UniProt
Western blot - Anti-VPS11 antibody (ab125083)
All lanes : Anti-VPS11 antibody (ab125083) at 0.04 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : Jurkat whole cell lysate at 50 µg Lane 4 : HeLa whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 108 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-VPS11 antibody (ab125083)
Detection of VPS11 by Western Blot of Immunprecipitate.
ab125083 at 0.4µg/ml staining VPS11 in 293T whole cell lysate immunoprecipitated using ab125083 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
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