概述

  • 产品名称
  • 描述
    兔多克隆抗体to VPS11
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 891-941 of Human VPS11 (NP_068375.3).

  • 阳性对照
    • 293T, Jurkat and HeLa whole cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab125083 was affinity purified using an epitope specific to VPS11 immobilized on solid support.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能
    May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
  • 组织特异性
    Ubiquitous. Expression was highest in heart and low in lung.
  • 序列相似性
    Belongs to the VPS11 family.
    Contains 1 clathrin repeat.
    Contains 1 RING-type zinc finger.
  • 细胞定位
    Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • END1 antibody
    • HGNC:14583 antibody
    • hVPS11 antibody
    • PEP5 antibody
    • PP3476 antibody
    • RING finger protein 108 antibody
    • RNF108 antibody
    • Vacuolar protein sorting 11 (yeast homolog) antibody
    • Vacuolar protein sorting 11 (yeast) antibody
    • Vacuolar protein sorting 11 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 11 homolog antibody
    • Vacuolar protein sorting associated protein 11 homolog antibody
    • Vacuolar protein sorting protein 11 antibody
    • Vacuolar protein sorting-associated protein 11 homolog antibody
    • vps11 antibody
    • VPS11_HUMAN antibody
    see all

Anti-VPS11 antibody 图像

  • All lanes : Anti-VPS11 antibody (ab125083) at 0.04 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 108 kDa


    Exposure time : 3 minutes
  • Detection of VPS11 by Western Blot of Immunprecipitate.
    ab125083 at 0.4µg/ml staining VPS11 in 293T whole cell lysate immunoprecipitated using ab125083 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-VPS11 antibody (ab125083)参考文献

This product has been referenced in:
  • Marwaha R  et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). Read more (PubMed: 28325809) »
  • Khatter D  et al. The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes. J Cell Sci 128:1746-61 (2015). Read more (PubMed: 25908847) »

See all 2 Publications for this product

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