Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human VPREB3.
- This antibody gave a positive control in Raji and Ramos whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
Associates with the Ig-mu chain to form a molecular complex that is expressed on the surface of pre-B-cells.
Expressed in B-cell precursors. Expressed in fetal liver, bone marrow, spleen and lymph node.
Belongs to the immunoglobulin superfamily.
Contains 1 Ig-like (immunoglobulin-like) domain.
Information by UniProt
- N27C7 2 antibody
- N27C7-2 antibody
- Pre B lymphocyte protein 3 antibody
Western blot - Anti-VPREB3 antibody (ab88487)
All lanes :
Anti-VPREB3 antibody (ab88487) at 1 µg/mlLane 1 :
Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 2 :
Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.SecondaryAll lanes :
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
14 kDaObserved band size:
14 kDaAdditional bands at:
110 kDa. We are unsure as to the identity of these extra bands.Exposure time:
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"