概述

  • 产品名称Anti-VPRBP抗体
    参阅全部 VPRBP 一抗
  • 描述
    兔多克隆抗体to VPRBP
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Horse, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human VPRBP.

    (Peptide available as ab86242.)

  • 阳性对照
    • This antibody gave a positive signal in Rat and Mouse Liver Tissue Lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 169 kDa (predicted molecular weight: 169 kDa).

靶标

  • 相关性VprBP may act as a receptor for CUL4-DDB1 ubiquitin ligase complex. In case of HIV1 infection, this protein is recruited by HIV1 VPR protein which is believed to result in an arrest of the cell cyccle in the G2 phase.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • DCAF1 antibody
    • DDB1 and CUL4 associated factor 1 antibody
    • HIV-1 Vpr-binding protein antibody
    • KIAA0800 antibody
    • protein VPRBP antibody
    • RIP antibody
    • Vpr (HIV-1) binding protein antibody
    • VPR BP antibody
    • Vpr interacting protein antibody
    • Vpr-binding protein antibody
    see all

Anti-VPRBP antibody 图像

  • All lanes : Anti-VPRBP antibody (ab75458) at 1 µg/ml

    Lane 1 : Liver (Rat) Tissue Lysate
    Lane 2 : : Liver (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 169 kDa
    Observed band size : 169 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab75458 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75458, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% PFA fixed (10 min) HeLa and MCF7 cells at 1µg/ml.
  • IHC image of VPRBP staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75458 at 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-VPRBP antibody (ab75458)参考文献

This product has been referenced in:
  • Cassiday PA  et al. Understanding the molecular manipulation of DCAF1 by the lentiviral accessory proteins Vpr and Vpx. Virology 476:19-25 (2015). Read more (PubMed: 25499532) »
  • Kogan M  et al. Inhibition of NF-?B activity by HIV-1 Vpr is dependent on Vpr binding protein. J Cell Physiol : (2012). WB ; Human . Read more (PubMed: 23001849) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab75458.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"