Full length native protein (purified) corresponding to VPH1. Purified S. cerevisiae vacuole membranes
This antibody clone is manufactured by Abcam.
This IgG2a kappa isotype control monoclonal antibody was raised in mouse against a yeast-specific protein, V-ATPase subunit (the product of VPH1 gene). It is a suitable isotype control for mammalian species.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact firstname.lastname@example.org or you can find further information here.
Shipped at 4°C. Store at +4°C.
Preservative: 0.02% Sodium azide Constituent: HBS
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Immunogen affinity purified
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Use a concentration of 20 µg/ml. Heat induced antigen retrieval recommended.
Subunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Simpkins JA et al. Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration. Biol Open5:689-97 (2016).
Read more (PubMed: 27142334) »