Anti-Von Willebrand Factor抗体(ab96340)

概述

  • 产品名称Anti-Von Willebrand Factor抗体
    参阅全部 Von Willebrand Factor 一抗
  • 描述
    兔多克隆抗体to Von Willebrand Factor
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1100 - 1200 of Human Von Willebrand Factor.

  • 阳性对照
    • This antibody gave a positive signal in Human spleen tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab96340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 275 kDa (predicted molecular weight: 309 kDa).

靶标

  • 功能Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 组织特异性Plasma.
  • 疾病相关Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • 序列相似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • 结构域The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • 翻译后修饰All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • 细胞定位Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Coagulation factor VIII antibody
    • Coagulation factor VIII VWF antibody
    • F8VWF antibody
    • Factor VIII related antigen antibody
    • von Willebrand antigen 2 antibody
    • von Willebrand antigen II antibody
    • Von Willebrand disease antibody
    • VWD antibody
    • vWF antibody
    • VWF_HUMAN antibody
    see all

Anti-Von Willebrand Factor antibody 图像

  • Anti-Von Willebrand Factor antibody (ab96340) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 309 kDa
    Observed band size : 275 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa,40 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Von Willebrand Factor antibody (ab96340)参考文献

ab96340 has not yet been referenced specifically in any publications.

Product Wall

The following antibodies could be suitable as per your request;

http://www.abcam.com/von-willebrand-factor-antibody-ab96340.html

http://www.abcam.com/von-willebrand-factor-antibody-aminoterminal-end-ab47129.html

Thank you for enquiry.

I am sorry to confirm that asfar as we are aware, ab96340 has never been tested in IHC-P. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our ...

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Vielen Dank für Ihren Anruf.

Nachdem ich wie versprochen alle unsere Von Willebrand Faktor- Antikörperdaraufhin untersucht habe, ob sie möglicherweise die Bindung von Faktor VIII inhibieren können, weiß ich nicht, ob...

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As far as I can see ab96340 should not cross react with Factor VIII. I blasted the immunogen sequence for ab96430 and only vWF came back. I also aligned the immunogen sequence to human factor VIII and there was no significant similarity.

If t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"