Anti-Von Willebrand Factor抗体(ab6994)

概述

  • 产品名称Anti-Von Willebrand Factor抗体
    参阅全部 Von Willebrand Factor 一抗
  • 描述
    兔多克隆抗体to Von Willebrand Factor
  • 特异性The product specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. Historically, this antibody worked well in mouse (see references), however more recent batches have shown inconsistent results in mouse. Therefore, we do not recommend this antibody for use in this species.
  • 经测试应用适用于: ICC/IF, IHC-FrFl, IHC-P, IHC-Fr, WB, Flow Cyt, IHC-FoFrmore details
  • 种属反应性
    与反应: Rat, Guinea pig, Cow, Dog, Human, Pig
    不与反应: Chicken
  • 免疫原

    Full length native protein (purified) corresponding to Human Von Willebrand Factor. Purified from plasma.

  • 阳性对照
    • Human tongue.

性能

应用

Our Abpromise guarantee covers the use of ab6994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/400.
IHC-FrFl 1/200. (see Abreview)
IHC-P 1/200 - 1/400. for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol. Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary. Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification.
IHC-Fr 1/1000.
WB 1/500.
Flow Cyt Use a concentration of 5 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration. PubMed: 19622235

靶标

  • 功能Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 组织特异性Plasma.
  • 疾病相关Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • 序列相似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • 结构域The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • 翻译后修饰All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • 细胞定位Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Coagulation factor VIII antibody
    • Coagulation factor VIII VWF antibody
    • F8VWF antibody
    • Factor VIII related antigen antibody
    • von Willebrand antigen 2 antibody
    • von Willebrand antigen II antibody
    • Von Willebrand disease antibody
    • VWD antibody
    • vWF antibody
    • VWF_HUMAN antibody
    see all

Anti-Von Willebrand Factor antibody 图像

  • Ab6994 positively staining formaldehyde fixed paraffin embedded mouse tumour sections (1/1000).Mice were subcutaneously injected with 3t3 cells which over expressed HER2.

    Secondary: Biotin conjugated horse anti rabbit (1/300).Detection was achieved using DAB and the sections were counterstained with Hematoxylin.

    This image is courtesy of an Abreview submitted by Jiqiang Zhang on 16 September 2005. for further details relating to the reviewers protocol please refer to the Abreviews section of the data sheet.

    See Abreview

  • Confocal extended focus photograph of von Willebrand factor staining (red) and non-specific nuclear counterstain (green) in the adult mouse olfactory bulb.  This picture shows a single major vessel within the glomerular layer of the olfactory bulb.  For more details see the review of this antibody by Adam Puche.

  • Immunocytochemical analysis labeling Von Willebrand Factor with ab6994 at 1/100 dilution.

    The nuclear counterstain is DAPI(blue)

  • ab6994 staining Von Willebrand Factor in pig skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was enzymatic using pronase, 1mg/ml. Samples were incubated with primary antibody (1/500 in PBS) for 12 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • This picture shows Factor VII Immunohistochemical localization in BCG infected Guinea Pig Lung. The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.

Anti-Von Willebrand Factor antibody (ab6994)参考文献

This product has been referenced in:
  • Schuhmann MK  et al. Immunohistochemical Analysis of Cerebral Thrombi Retrieved by Mechanical Thrombectomy from Patients with Acute Ischemic Stroke. Int J Mol Sci 17:N/A (2016). IHC-P ; Human . Read more (PubMed: 26927082) »
  • Li SH  et al. VEGF and FGF2 Improve Revascularization, Survival, and Oocyte Quality of Cryopreserved, Subcutaneously-Transplanted Mouse Ovarian Tissues. Int J Mol Sci 17:N/A (2016). IHC-P ; Mouse . Read more (PubMed: 27483256) »

See all 135 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Spinal cord)
Antigen retrieval step None
Permeabilization Yes - Triton-X100
Specification Spinal cord
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative Paraformaldehyde
Username

清隆 新井

Verified customer

提交于 Sep 30 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (kidney)
Antigen retrieval step None
Permeabilization No
Specification kidney
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Miss. jin young park

Verified customer

提交于 Aug 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - 0.3% triton-x-100
Specification Brain
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 15 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (Cerebellum)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization No
Specification Cerebellum
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative Formaldehyde
Username

清隆 新井

Verified customer

提交于 Aug 21 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Hamster Tissue sections (kidney from syrian hamster)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Pronase 1mg/ml
Permeabilization No
Specification kidney from syrian hamster
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 13 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (skin)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Pronase 1mg/ml
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 27 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Thymus)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Pronase 1mg/ml
Permeabilization No
Specification Thymus
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 27 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreas)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Pronase 1mg/ml
Permeabilization No
Specification pancreas
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 27 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dasypus novemcinctus Tissue sections (Uterus (non-pregnant))
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, pH 6.0
Permeabilization No
Specification Uterus (non-pregnant)
Blocking step BSA as blocking agent for 5 minute(s) · Concentration: 0.1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Arun Chavan

Verified customer

提交于 Jul 24 2015

Application Flow Cytometry
Fixation 4% Paraformaldehyde for 20 minutes
Permeabilization Yes - Saponin, commercial prep
Sample Rat Cell (Rat Aortic Endothelial Cells)
Specification Rat Aortic Endothelial Cells
Preparation Cell harvesting/tissue preparation method: Use 1 million cells/reaction
Sample buffer: Commercial prep, composition unknown
Username

Abcam user community

Verified customer

提交于 Oct 13 2014

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