Anti-Von Willebrand Factor抗体(ab6994)

概述

  • 产品名称
    Anti-Von Willebrand Factor抗体
    参阅全部 Von Willebrand Factor 一抗
  • 描述
    兔多克隆抗体to Von Willebrand Factor
  • 特异性
    The product specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. Historically, this antibody worked well in mouse (see references), however more recent batches have shown inconsistent results in mouse. Therefore, we do not recommend this antibody for use in this species.
  • 经测试应用
    适用于: ICC/IF, IHC-FrFl, IHC-P, IHC-Fr, WB, Flow Cyt, IHC-FoFrmore details
  • 种属反应性
    与反应: Rat, Sheep, Guinea pig, Cow, Dog, Human, Pig
    不与反应: Chicken
  • 免疫原

    Full length native protein (purified) corresponding to Human Von Willebrand Factor. Purified from plasma.

  • 阳性对照
    • Human tongue.

性能

应用

Our Abpromise guarantee covers the use of ab6994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/400.
IHC-FrFl 1/200. (see Abreview)
IHC-P 1/200 - 1/400. for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol. Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary. Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification.
IHC-Fr 1/1000.
WB 1/500.
Flow Cyt Use a concentration of 5 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-FoFr Use at an assay dependent concentration. PubMed: 19622235

靶标

  • 功能
    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 组织特异性
    Plasma.
  • 疾病相关
    Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • 序列相似性
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • 结构域
    The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • 翻译后修饰
    All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • 细胞定位
    Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Coagulation factor VIII antibody
    • Coagulation factor VIII VWF antibody
    • F8VWF antibody
    • Factor VIII related antigen antibody
    • von Willebrand antigen 2 antibody
    • von Willebrand antigen II antibody
    • Von Willebrand disease antibody
    • VWD antibody
    • vWF antibody
    • VWF_HUMAN antibody
    see all

Anti-Von Willebrand Factor antibody 图像

  • Ab6994 positively staining formaldehyde fixed paraffin embedded mouse tumour sections (1/1000).Mice were subcutaneously injected with 3t3 cells which over expressed HER2.

    Secondary: Biotin conjugated horse anti rabbit (1/300).Detection was achieved using DAB and the sections were counterstained with Hematoxylin.

    This image is courtesy of an Abreview submitted by Jiqiang Zhang on 16 September 2005. for further details relating to the reviewers protocol please refer to the Abreviews section of the data sheet.

    See Abreview

  • Confocal extended focus photograph of von Willebrand factor staining (red) and non-specific nuclear counterstain (green) in the adult mouse olfactory bulb.  This picture shows a single major vessel within the glomerular layer of the olfactory bulb.  For more details see the review of this antibody by Adam Puche.

  • Immunocytochemical analysis labeling Von Willebrand Factor with ab6994 at 1/100 dilution.

    The nuclear counterstain is DAPI(blue)

  • ab6994 staining Von Willebrand Factor in pig skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was enzymatic using pronase, 1mg/ml. Samples were incubated with primary antibody (1/500 in PBS) for 12 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • This picture shows Factor VII Immunohistochemical localization in BCG infected Guinea Pig Lung. The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.

Anti-Von Willebrand Factor antibody (ab6994)参考文献

This product has been referenced in:
  • Xu L  et al. Umbilical cord-derived mesenchymal stem cells on scaffolds facilitate collagen degradation via upregulation of MMP-9 in rat uterine scars. Stem Cell Res Ther 8:84 (2017). IHC ; Rat . Read more (PubMed: 28420433) »
  • Montali M  et al. Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation. Stem Cell Res Ther 8:106 (2017). ICC/IF ; Human . Read more (PubMed: 28464921) »

See all 150 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Kidney)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Protease 2 (Ventana)
Permeabilization
No
Specification
Kidney
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 31 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - Triton-X100
Specification
Spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde
Username

清隆 新井

Verified customer

提交于 Sep 30 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (kidney)
Antigen retrieval step
None
Permeabilization
No
Specification
kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Miss. Jin Young Park

Verified customer

提交于 Aug 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% triton-x-100
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 15 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (Cerebellum)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Permeabilization
No
Specification
Cerebellum
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Formaldehyde
Username

清隆 新井

Verified customer

提交于 Aug 21 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Hamster Tissue sections (kidney from syrian hamster)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Pronase 1mg/ml
Permeabilization
No
Specification
kidney from syrian hamster
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 13 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (skin)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Pronase 1mg/ml
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 27 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Thymus)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Pronase 1mg/ml
Permeabilization
No
Specification
Thymus
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 27 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Pronase 1mg/ml
Permeabilization
No
Specification
pancreas
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 27 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dasypus novemcinctus Tissue sections (Uterus (non-pregnant))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, pH 6.0
Permeabilization
No
Specification
Uterus (non-pregnant)
Blocking step
BSA as blocking agent for 5 minute(s) · Concentration: 0.1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Arun Chavan

Verified customer

提交于 Jul 24 2015

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