Anti-Von Willebrand Factor抗体(ab11713)

概述

  • 产品名称
    Anti-Von Willebrand Factor抗体
    参阅全部 Von Willebrand Factor 一抗
  • 描述
    羊多克隆抗体to Von Willebrand Factor
  • 特异性
    ab11713 detects Human von Willebrand factor.
  • 经测试应用
    适用于: IHC-Fr, Immunodiffusion, Flow Cyt, ELISA, ICC/IFmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Human, Pig
  • 免疫原

    Purified human von Willebrand factor.

  • 阳性对照
    • Tonsil.
  • 常规说明
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab11713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/50 - 1/100.
Immunodiffusion 1/1.
Flow Cyt Use at an assay dependent concentration.

ab37385 - Sheep polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能
      Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
    • 组织特异性
      Plasma.
    • 疾病相关
      Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
    • 序列相似性
      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 3 VWFA domains.
      Contains 3 VWFC domains.
      Contains 4 VWFD domains.
    • 结构域
      The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
    • 翻译后修饰
      All cysteine residues are involved in intrachain or interchain disulfide bonds.
      N- and O-glycosylated.
    • 细胞定位
      Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Coagulation factor VIII antibody
      • Coagulation factor VIII VWF antibody
      • F8VWF antibody
      • Factor VIII related antigen antibody
      • von Willebrand antigen 2 antibody
      • von Willebrand antigen II antibody
      • Von Willebrand disease antibody
      • VWD antibody
      • vWF antibody
      • VWF_HUMAN antibody
      see all

    图片

    • ab11713 at a 1/1000 dilution staining Von Willebrand Factor in mouse blood vessels by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized with 0.1% Triton. Blocked using 20% serum for 1 hour at 19°C. Secondary used at 1/200 polyclonal Donkey anti-sheep conjugated to Alexa Fluor 647 (pink). Counterstain (blue) DAPI.

      See Abreview

    • ab11713 staining Von Willebrand Factor in porcine heart tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in acetone, permeabilized using 0.3% Triton/PBS, blocked with 10% serum for 30 minutes at 22°C and then incubated with ab11713 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a FITC conjugated donkey anti-sheep polyclonal used at a 1/1000 dilution.

      See Abreview

    文献

    This product has been referenced in:
    • Yurdagul A  et al. Oxidized LDL induces FAK-dependent RSK signaling to drive NF-?B activation and VCAM-1 expression. J Cell Sci 129:1580-91 (2016). Read more (PubMed: 26906414) »
    • Claude C  et al. Inhalable delivery of AAV-based MRP4/ABCC4 silencing RNA prevents monocrotaline-induced pulmonary hypertension. Mol Ther Methods Clin Dev 2:14065 (2015). Read more (PubMed: 26052533) »

    See all 18 Publications for this product

    客户评价及客户问答

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Keloid)
    Permeabilization
    No
    Specification
    Keloid
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    提交于 Sep 15 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Lung)
    Permeabilization
    Yes - Triton 0.1%
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Zinc fixative
    Username

    Abcam user community

    Verified customer

    提交于 Jul 25 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (A7r5 smooth muscle cells)
    Permeabilization
    Yes - Triton and Tween
    Specification
    A7r5 smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Formaldehyde
    Username

    Julie Kohn

    Verified customer

    提交于 May 02 2016

    Application
    Immunocytochemistry
    Sample
    Guinea pig Cell (Endothelilal cells freshly isolated)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Endothelilal cells freshly isolated
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Sep 23 2015

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skin)
    Gel Running Conditions
    Reduced Denaturing (7.5%gel)
    Loading amount
    125 µg
    Treatment
    Wound
    Specification
    Skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 27 2015

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (brain, liver)
    Specification
    brain, liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 05 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (skin dermis)
    Specification
    skin dermis
    Permeabilization
    No
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    提交于 May 30 2014

    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% saponin
    Sample
    Mouse Cell (skin)
    Specification
    skin
    Preparation
    Cell harvesting/tissue preparation method: Wilson L, Fathke C, Isik F. Tissue dispersion and flow cytometry for the cellular analysis of wound healing. BioTechniques 2002: 32: 548-551.
    Sample buffer: 2%BSA/PBS
    Username

    Abcam user community

    Verified customer

    提交于 May 14 2014

    Thank you for contacting Abcam.

    I ran an alignment of the human and chicken VWF protein sequence, and there is only 56.1% homology between the two. Our AbTrial program, which allows researchers to test our products in untested species and rece...

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    Thank you very much for your call today and for your patience while I've been in touch with the production lab regarding your enquiry.
    The IgG concentration of this lot is 9.13 mg/ml.
    I hope that this information will be useful, but if you ha...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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