Anti-Von Willebrand Factor抗体[4F9] (ab20435)


  • 产品名称Anti-Von Willebrand Factor抗体[4F9]
    参阅全部 Von Willebrand Factor 一抗
  • 描述
    小鼠单克隆抗体[4F9] to Von Willebrand Factor
  • 经测试应用适用于: ICC/IF, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    FVIII: C/vWF complex (denaturated)



Our Abpromise guarantee covers the use of ab20435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 22280443
Flow Cyt Use 2µg for 105 cells. . ab20435 has been used in flow cytometry with Jurkat cells and with platelets. The Jurkat cell line was fixed using 1% formaldehyde followed by permeabilization using a commercially-available saponin reagent for permeabilization. The platelets were fixed using 1% formaldehyde, washed and suspended in PBS without permeabilization.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/50 - 1/100.


  • 功能Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 组织特异性Plasma.
  • 疾病相关Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • 序列相似性Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • 结构域The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • 翻译后修饰All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • 细胞定位Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Coagulation factor VIII antibody
    • Coagulation factor VIII VWF antibody
    • F8VWF antibody
    • Factor VIII related antigen antibody
    • von Willebrand antigen 2 antibody
    • von Willebrand antigen II antibody
    • Von Willebrand disease antibody
    • VWD antibody
    • vWF antibody
    • VWF_HUMAN antibody
    see all

Anti-Von Willebrand Factor antibody [4F9] 图像

  • ab20435, staining Von Willebrand Factor (green) in hMSC-TERT, hADMSC, hADSSC and hNFSSC cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100 for 10 min. After washing with PBS, cells were blocked with 3% BSA for 30 min and incubated with primary antibody at 10 µl/ml, at 4°C overnight. A FITC-conjugated goat anti-mouse polyclonal IgG (1/4000) was used as the secondary antibody.

Anti-Von Willebrand Factor antibody [4F9] (ab20435)参考文献

This product has been referenced in:
  • Masuda H  et al. Vasculogenic conditioning of peripheral blood mononuclear cells promotes endothelial progenitor cell expansion and phenotype transition of anti-inflammatory macrophage and T lymphocyte to cells with regenerative potential. J Am Heart Assoc 3:e000743 (2014). Read more (PubMed: 24965023) »
  • Vishnubalaji R  et al. In vitro differentiation of human skin-derived multipotent stromal cells into putative endothelial-like cells. BMC Dev Biol 12:7 (2012). ICC/IF ; Human . Read more (PubMed: 22280443) »

See all 3 Publications for this product

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Nachdem ich wie versprochen alle unsere Von Willebrand Faktor- Antikörperdaraufhin untersucht habe, ob sie möglicherweise die Bindung von Faktor VIII inhibieren können, weiß ich nicht, ob...

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Merci de nous avoir contactés. Le laboratoire m'a informé que l'anticorps ab20435 a été utilisé en cytométrie en flux sur cellules Jurkat et thrombocytes. - Cellules Jurkat : fixation avec 1% formaldéhyde et perméabilisation avec une sol...

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Thank you for your enqiry regarding the FACS testing of this antibody (ab20435). The flow cytometry application for this antibody is in a published reference. The full text is available free on-line at

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