Anti-Von Willebrand Factor抗体[3E2D10] (ab194405)
Key features and details
- Mouse monoclonal [3E2D10] to Von Willebrand Factor
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Von Willebrand Factor抗体[3E2D10]
参阅全部 Von Willebrand Factor 一抗 -
描述
小鼠单克隆抗体[3E2D10] to Von Willebrand Factor -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human Von Willebrand Factor aa 800-950.
Database link: P04275 -
阳性对照
- IHC-P: Human pancreas and tonsil tissues.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from Bioreactor Concentrate -
克隆
单克隆 -
克隆编号
3E2D10 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab194405于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
use 10mM Tris with 1mM EDTA, pH 9.0 for antigen retrieval. |
说明 |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. use 10mM Tris with 1mM EDTA, pH 9.0 for antigen retrieval. |
靶标
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功能
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
组织特异性
Plasma. -
疾病相关
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
序列相似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
结构域
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
翻译后修饰
All cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
细胞定位
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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数据库链接
- Entrez Gene: 7450 Human
- Omim: 613160 Human
- SwissProt: P04275 Human
- Unigene: 440848 Human
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别名
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling Von Willebrand Factor with ab194405 at 2 µg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Von Willebrand Factor with ab194405 at 1.0 µg/mL.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab194405 被引用在 10 文献中.
- Zhang SX et al. Mechanism of N-Methyl-N-Nitroso-Urea-Induced Gastric Precancerous Lesions in Mice. J Oncol 2022:3780854 (2022). PubMed: 35342404
- Valdebenito S et al. COVID-19 Lung Pathogenesis in SARS-CoV-2 Autopsy Cases. Front Immunol 12:735922 (2021). PubMed: 34671353
- Liang L et al. Exosomes derived from miR-301a-3p-overexpressing adipose-derived mesenchymal stem cells reverse hypoxia-induced erectile dysfunction in rat models. Stem Cell Res Ther 12:87 (2021). PubMed: 33494812
- Liang Y et al. Inhibition of Caspase-1 Ameliorates Ischemia-Associated Blood-Brain Barrier Dysfunction and Integrity by Suppressing Pyroptosis Activation. Front Cell Neurosci 14:540669 (2020). PubMed: 33584203
- Chang Z et al. Snail promotes the generation of vascular endothelium by breast cancer cells. Cell Death Dis 11:457 (2020). PubMed: 32541667
- Chen C et al. Endothelial transdifferentiation of human HGC-27 gastric cancer cells in vitro. Oncol Lett 20:303 (2020). PubMed: 33093912
- Ito Y et al. Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex Vivo Production. Cell 174:636-648.e18 (2018). PubMed: 30017246
- Klotz BJ et al. Engineering of a complex bone tissue model with endothelialised channels and capillary-like networks. Eur Cell Mater 35:335-348 (2018). PubMed: 29873804
- Garbuzova-Davis S et al. Endothelial and Astrocytic Support by Human Bone Marrow Stem Cell Grafts into Symptomatic ALS Mice towards Blood-Spinal Cord Barrier Repair. Sci Rep 7:884 (2017). IHC ; Mouse . PubMed: 28408761
- Yue X et al. Endothelial lipase is upregulated by interleukin-6 partly via the p38 MAPK and p65 NF-?B signaling pathways. Mol Med Rep 14:1979-85 (2016). Human . PubMed: 27430252