Anti-VKORC1抗体(ab108053)

概述

  • 产品名称Anti-VKORC1抗体
    参阅全部 VKORC1 一抗
  • 描述
    兔多克隆抗体to VKORC1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide corresponding to a region between N terminal amino acids 22-49 of Human VKORC1 (NP_076869.1).

  • 阳性对照
    • NCI-H460 cell line lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab108053 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab108053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K.
  • 组织特异性Expressed at highest levels in fetal and adult liver, followed by fetal heart, kidney, and lung, adult heart, and pancreas.
  • 疾病相关Defects in VKORC1 are a cause of combined deficiency of vitamin K-dependent clotting factors type 2 (VKCFD2) [MIM:607473]. VKCFD leads to a bleeding tendency that is usually reversed by oral administration of vitamin K.
    Defects in VKORC1 are a cause of coumarin resistance (CMRES) [MIM:122700]; also known as warfarin resistance. Warfarin and other coumarin-type anticoagulants are used to reduce blood viscosity in the treatment of thromboembolic disorders.
  • 序列相似性Belongs to the VKOR family.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3-epoxide reductase subunit 1 antibody
    • EDTP308 antibody
    • FLJ00289 antibody
    • IMAGE3455200 antibody
    • MGC2694 antibody
    • MST134 antibody
    • MST576 antibody
    • Phylloquinone epoxide reductase antibody
    • Vitamin K dependent clotting factors deficiency 2 antibody
    • Vitamin K epoxide reductase complex subunit 1 antibody
    • Vitamin K1 2 antibody
    • Vitamin K1 2,3-epoxide reductase subunit 1 antibody
    • Vitamin K1 epoxide reductase (warfarin sensitive) antibody
    • VKCFD2 antibody
    • VKOR antibody
    • VKOR1_HUMAN antibody
    • VKORC1 antibody
    see all

Anti-VKORC1 antibody 图像

  • Anti-VKORC1 antibody (ab108053) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 18 kDa
  • ab108053, at a 1/50 dilution, staining VKORC1 in formalin fixed and paraffin embedded Human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-VKORC1 antibody (ab108053)参考文献

ab108053 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Primary hepatocyte culture)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Specification Primary hepatocyte culture
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Jul 29 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Sample Human Cell lysate - whole cell (HEK293, Y79, HepG2, A549)
Specification HEK293, Y79, HepG2, A549
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jul 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"