Anti-VKORC1抗体(ab108053)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to VKORC1
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A KLH conjugated synthetic peptide corresponding to a region between N terminal amino acids 22-49 of Human VKORC1 (NP_076869.1).

  • 阳性对照
    • NCI-H460 cell line lysates

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab108053 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab108053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K.
  • 组织特异性
    Expressed at highest levels in fetal and adult liver, followed by fetal heart, kidney, and lung, adult heart, and pancreas.
  • 疾病相关
    Defects in VKORC1 are a cause of combined deficiency of vitamin K-dependent clotting factors type 2 (VKCFD2) [MIM:607473]. VKCFD leads to a bleeding tendency that is usually reversed by oral administration of vitamin K.
    Defects in VKORC1 are a cause of coumarin resistance (CMRES) [MIM:122700]; also known as warfarin resistance. Warfarin and other coumarin-type anticoagulants are used to reduce blood viscosity in the treatment of thromboembolic disorders.
  • 序列相似性
    Belongs to the VKOR family.
  • 细胞定位
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3-epoxide reductase subunit 1 antibody
    • EDTP308 antibody
    • FLJ00289 antibody
    • IMAGE3455200 antibody
    • MGC2694 antibody
    • MST134 antibody
    • MST576 antibody
    • Phylloquinone epoxide reductase antibody
    • Vitamin K dependent clotting factors deficiency 2 antibody
    • Vitamin K epoxide reductase complex subunit 1 antibody
    • Vitamin K1 2 antibody
    • Vitamin K1 2,3-epoxide reductase subunit 1 antibody
    • Vitamin K1 epoxide reductase (warfarin sensitive) antibody
    • VKCFD2 antibody
    • VKOR antibody
    • VKOR1_HUMAN antibody
    • VKORC1 antibody
    see all

图片

  • Anti-VKORC1 antibody (ab108053) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 18 kDa
  • ab108053, at a 1/50 dilution, staining VKORC1 in formalin fixed and paraffin embedded Human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

文献

ab108053 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (Primary hepatocyte culture)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Primary hepatocyte culture
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jul 29 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Cell lysate - whole cell (HEK293, Y79, HepG2, A549)
Specification
HEK293, Y79, HepG2, A549
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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