Anti-Vitronectin抗体[VN58-1] (ab13413)

概述

  • 产品名称Anti-Vitronectin抗体[VN58-1]
    参阅全部 Vitronectin 一抗
  • 描述
    小鼠单克隆抗体[VN58-1] to Vitronectin
  • 特异性This antibody does not interfere with vitronectin-mediated adhesion.
  • 经测试应用适用于: ICC/IF, ELISA, WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
    不与反应: Cow
  • 免疫原

    Human Vitronectin.

  • 表位This antibody specifically reacts with an epitope in the region of amino acids 1-130 of human vitronectin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号VN58-1
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab13413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/3000. (with solid phase antigen)
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 5 - 10 µg/ml.
IHC-Fr Use a concentration of 5 - 10 µg/ml.

靶标

  • 功能Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • 组织特异性Plasma.
  • 序列相似性Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • 结构域The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • 翻译后修饰Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位Secreted, extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Complement S Protein antibody
    • Epibolin antibody
    • S Protein antibody
    • S-protein antibody
    • Serum Spreading Factor antibody
    • Serum-spreading factor antibody
    • Somatomedin B antibody
    • Somatomedin-B antibody
    • V75 antibody
    • Vitronectin V10 subunit antibody
    • Vitronectin V65 subunit antibody
    • VN antibody
    • VNT antibody
    • VTN antibody
    • VTNC_HUMAN antibody
    see all

Anti-Vitronectin antibody [VN58-1] 图像

  • This picture shows formalin-fixed paraffin embedded human skin stained with Vitronectin (1:100 - 30 minutes RT). The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.
  • ICC/IF image of ab13413 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13413, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Vitronectin antibody [VN58-1] (ab13413)参考文献

This product has been referenced in:
  • Zhou J  et al. Direct role of interrod spacing in mediating cell adhesion on Sr-HA nanorod-patterned coatings. Int J Nanomedicine 9:1243-60 (2014). ICC/IF ; Human . Read more (PubMed: 24634585) »
  • Stepanek O  et al. Interaction of late apoptotic and necrotic cells with vitronectin. PLoS One 6:e19243 (2011). Read more (PubMed: 21573223) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Epithelial OVCA cell line)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 100 µg
Treatment Perifosine 24 hr
Specification Epithelial OVCA cell line
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Aug 12 2016

Thank you for getting back to me with those details. Having reviewed your protocol there are a few things I may be able to suggest. The dilution for ab13413 is recommended in the range of 5-10µg/ml and ab27710 at an assay dependent dilution. I ...

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Thank you for contacting us. I am sorry that you are experiencing problems with these antibodies. In order to assist you as best I can could you please fill in the questionnaire I have attached to this email. This will allow me to understand more ...

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