Anti-Vitronectin抗体[EP873Y] (ab45139)


  • 产品名称Anti-Vitronectin抗体[EP873Y]
    参阅全部 Vitronectin 一抗
  • 描述
    兔单克隆抗体[EP873Y] to Vitronectin
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the V10 subunit of human Vitronectin.

  • 阳性对照
    • Human liver tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Vitronectin antibody (HRP) [EP873Y] (ab199310)



Our Abpromise guarantee covers the use of ab45139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 75 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
      Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
    • 组织特异性Plasma.
    • 序列相似性Contains 4 hemopexin repeats.
      Contains 1 SMB (somatomedin-B) domain.
    • 结构域The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
    • 翻译后修饰Sulfated on 2 tyrosine residues.
      N- and O-glycosylated.
      Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
      It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
      Phosphorylation sites are present in the extracellular medium.
    • 细胞定位Secreted, extracellular space.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Complement S Protein antibody
      • Epibolin antibody
      • S Protein antibody
      • S-protein antibody
      • Serum Spreading Factor antibody
      • Serum-spreading factor antibody
      • Somatomedin B antibody
      • Somatomedin-B antibody
      • V75 antibody
      • Vitronectin V10 subunit antibody
      • Vitronectin V65 subunit antibody
      • VN antibody
      • VNT antibody
      • VTN antibody
      • VTNC_HUMAN antibody
      see all

    Anti-Vitronectin antibody [EP873Y] 图像

    • Anti-Vitronectin antibody [EP873Y] (ab45139) at 1/20000 dilution + Hu Serum membrane at 10 µg

      Goat anti-rabbit HRP labelled, at 1/2000 dilution

      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • Ab45139 at a 1/100 dilution, staining human Vitronectin in human liver tissue by immunohistochemistry using paraffin embedded tissue.

    Anti-Vitronectin antibody [EP873Y] (ab45139)参考文献

    This product has been referenced in:
    • Piccolo SR  et al. Integrative analyses reveal signaling pathways underlying familial breast cancer susceptibility. Mol Syst Biol 12:860 (2016). WB ; Human . Read more (PubMed: 26969729) »
    • Kapinas K  et al. Bone matrix osteonectin limits prostate cancer cell growth and survival. Matrix Biol 31:299-307 (2012). WB . Read more (PubMed: 22525512) »

    See all 4 Publications for this product

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