RabMAb

Anti-Vitronectin抗体[EP781Y] (ab46808)

概述

  • 产品名称Anti-Vitronectin抗体[EP781Y]
    参阅全部 Vitronectin 一抗
  • 描述
    兔单克隆抗体[EP781Y] to Vitronectin
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the hemopexin-like 1 domain of human Vitronectin.

  • 阳性对照
    • Human serum; human lung tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 纯化说明This antibody has not been purified. It is provided in tissue culture supernatant and storage buffer.
  • 克隆单克隆
  • 克隆编号EP781Y
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab46808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 54,60,75 kDa (predicted molecular weight: 54 kDa).
IP 1/50.
IHC-P 1/100 - 1/250.

靶标

  • 功能Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • 组织特异性Plasma.
  • 序列相似性Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • 结构域The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • 翻译后修饰Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位Secreted, extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Complement S Protein antibody
    • Epibolin antibody
    • S Protein antibody
    • S-protein antibody
    • Serum Spreading Factor antibody
    • Serum-spreading factor antibody
    • Somatomedin B antibody
    • Somatomedin-B antibody
    • V75 antibody
    • Vitronectin V10 subunit antibody
    • Vitronectin V65 subunit antibody
    • VN antibody
    • VNT antibody
    • VTN antibody
    • VTNC_HUMAN antibody
    see all

Anti-Vitronectin antibody [EP781Y] 图像

  • Anti-Vitronectin antibody [EP781Y] (ab46808) at 1/20000 dilution + 10ug Human serum

    Secondary
    Goat anti-rabbit, HRP labelled. at 1/2000 dilution

    Predicted band size : 54 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa (possible non-specific binding),60 kDa (possible non-specific binding).
  • This image shows human lung tissue treated with ab46808 at 1/100 dilution.

Anti-Vitronectin antibody [EP781Y] (ab46808)参考文献

ab46808 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"